Sexing of mouse embryos by chromosomal analysis = 염색체 분석에 의한 생쥐 수정란의 성감별

Abstract

These experiments were carried out to obtain basic information necessary for sexing embryos by chromosomal analysis. To observe metaphase chromosomes, all embryos developed to blastocysts were cultured in Hoppe & Pitts’ medium containing 0.001% Colcemid under the gas phase of 5% CO! in air at 37 C for 2 hours. The sex chronjosome of mouse embryos shown normal development after culture in medium containing H-Y antiserum (10%，v/v) and complement (20%, v/v) also was confimed by chromosomal analysis. The results obtained in these experiments were summarized as follows： 1. Among 89 mouse blastocysts, the number of embryos identified to have XX and XY chromosome was 22(25%) and 25(28%), respectively and 42(47%) embryos were not identified. 2. Of total 40 mouse balstocysts cultured in medium containing H-Y antiserum and complement, 23(58%) embryos which were able to be discriminated their sex chromosomes were identified to be XX bearing embryos. 3. Sex chromosomes of a number of embryos subjected to chromosomal nalysis were not identified. This result may be due to absence or poor quality of metaphase spreads.