Effect of H-Y antibody on in vitro development of mouse embryos = H-Y 항체의 처리가 생쥐수정란의 발달에 미치는 영향

Abstract

These experiments were carried out to develop new techniques identifying XX-bearing embryos prior to implantation by immunological method. Antiserum to histocompatibility-Y(H-Y) antigen was prepared in adult SD(sprague-dawley) female rat by repeated immunization of new-bone testis supernatant from males of the same strain. ELISA test was used to identify the H-Y antibody of antiserum. Total 124 mouse embryos (8-cell stage) were treated with H-Y antiserum and complement in BSA free Hoppe and Pitt’s medium and cultured under the gas phase of 5% CO2 in air at 37°C for 24 to 48 hrs. The morphological chracteristics of embryos treated were observed under the phase-contrast microscope. The results obtained in these experiments were summwized as follows: 1. Optimal Density of H-Y antibody were appeared to be 0.27-0.47 by ELISA test. 2. Of total 124 embryos treated with H-Y antiserum and complement 69(55.6%) embryos developed to blastocyst and 55(44.4%) destroyed or arrested.