Production of interferon-gamma from human peripheral blood lymphocytes

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dc.contributor.authorIl Yup Chung-
dc.contributor.authorYoung Il Yeom-
dc.contributor.authorSang Ki Rhee-
dc.contributor.authorTai Wha Chung-
dc.contributor.authorMoon Hi Han-
dc.description.abstractInterferon- 7 (IFN- 7) was produced from human peripheral blood lymphocytes (Hu-PBL) by stimulating them with various kinds of mitogens. T-cell mitogens such as Con A and PHA-M were much more potent than B-cell mitogen such as PWM in producing IFN- 7 from Hu-PBL. The combined treatment of TPA with these mitogens enhanced IFN- / production ten fold compared with the single treatment of each mitogen. Both DNA synthesis and IFN- 7 production yielded bell-shaped dose-response curves for a given population of Hu-PBL in response to Con A. The effect of fetal calf serum(FCS) on IFN- 7 production was found to be critical: titer of IFN- 7 produced was very low in the absence of FCS and reached peak point at 2% FCS, beginning to decrease precipitously with an increasing FCS concentration up to 20%. Using the relationship between IFN- 7 production and mitogenic stimulation, it was found that one of factors responsible for the decrease of IFN- 7 production at the high serum concentration could be the binding of Con A to serum proteins which rendered Con A to be removed from the production system.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleProduction of interferon-gamma from human peripheral blood lymphocytes-
dc.title.alternativeProduction of interferon-gamma from human peripheral blood lymphocytes-
dc.citation.titleKorean Biochemical Journal-
dc.contributor.affiliatedAuthorYoung Il Yeom-
dc.contributor.affiliatedAuthorSang Ki Rhee-
dc.contributor.affiliatedAuthorTai Wha Chung-
dc.contributor.affiliatedAuthorMoon Hi Han-
dc.identifier.bibliographicCitationKorean Biochemical Journal, vol. 19, no. 3, pp. 219-224-
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Division of Biomedical Research > Personalized Genomic Medicine Research Center > 1. Journal Articles
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