Cloning of insecticidal crystal toxin gene from Bacillus thuringiensis subsp. kurstaki HD-73 and its toxicity to the fall webworm, Hyphantria cunea

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Title
Cloning of insecticidal crystal toxin gene from Bacillus thuringiensis subsp. kurstaki HD-73 and its toxicity to the fall webworm, Hyphantria cunea
Author(s)
So Young Min; Ho Yong Park; Myung Hee Kim; Jeong Il Kim
Bibliographic Citation
Korean Biochemical Journal, vol. 19, no. 4, pp. 363-371
Publication Year
1986
Abstract
Insecticidal crystal protein (CP) gene of Bacillus thuringiensis subsp. kurstaki HD-73 has been identified to be located in a 75-kilobase plasmid by southern hybridization technique using the biotinylated DNA probe (pC34), and cloned into the gram negative bacterium, Escherichia coli. E. coli transformants harboring full length or truncated (nearly full length) CP genes have expressed them with no significant differences in their larvicidal activity. The crude extracts of 五. coli clones were seen to be about 30-fold less potent than that of HD-73, as long as the larvicidal activity against the fall webworm, Hyphantria cunea, is concerned.
ISSN
0368-4881
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
Division of Biomedical Research > Microbiome Convergence Research Center > 1. Journal Articles
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