Cloning of insecticidal crystal toxin gene from Bacillus thuringiensis subsp. kurstaki HD-73 and its toxicity to the fall webworm, Hyphantria cunea
Cited 0 time in
- Cloning of insecticidal crystal toxin gene from Bacillus thuringiensis subsp. kurstaki HD-73 and its toxicity to the fall webworm, Hyphantria cunea
- So Young Min; Ho Yong Park; Myung Hee Kim; Jeong Il Kim
- Bibliographic Citation
- Korean Biochemical Journal, vol. 19, no. 4, pp. 363-371
- Publication Year
- Insecticidal crystal protein (CP) gene of Bacillus thuringiensis subsp. kurstaki HD-73 has been identified to be located in a 75-kilobase plasmid by southern hybridization technique using the biotinylated DNA probe (pC34), and cloned into the gram negative bacterium, Escherichia coli. E. coli transformants harboring full length or truncated (nearly full length) CP genes have expressed them with no significant differences in their larvicidal activity. The crude extracts of 五. coli clones were seen to be about 30-fold less potent than that of HD-73, as long as the larvicidal activity against the fall webworm, Hyphantria cunea, is concerned.
- Korea Soc-Assoc-Inst
- Appears in Collections:
- Division of Biomedical Research > Microbiome Convergence Research Center > 1. Journal Articles
- Files in This Item:
Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.