Sexing mouse demi-embryos by chromosomal analysis = 염색체 분석에 의한 생쥐 분할란의 성감별

Abstract

These experiments were carried out to obtain basic information necessary to produce the offspring which were sexed by chromosomal analysis prior to transfer in the mouse. Morula stage embryos were obtained at 3 days post-coitum. Embryos were mechanically bisected by a glass microneedle and then the pairs of demi-embryos were cultured in BMOC-3 medium under the humidified atmosphere of 5 % CO2 at 37 deg C for 22-26 hrs. To increase the appearance of metaphase, bisected embryos normally developed to the blastocyst were cultured in MOC-3 medium containing colcemid (0.04 micro g/ml) under the same condition for 3 hrs