Induction of parthenogenetic eggs by ethanol treatment in mouse = 생쥐에 있어서 ethanol처리에 의한 단위발생의 유기

Abstract

These experiments were carried out to obtain the basic informations and to establish the best conditions needed for the activation of mouse eggs by ethanol treatment. The influence of ethanol concentration and the age of eggs, time from hCG injection to recovery, on activation frequency of eggs was investigated. To activate the mouse eggs parthenogenetically, cumulus masses including eggs were released into a fresh solution of ethanol for 5 min and then rinsed out 5-6 times with ethanol-free medium. After incubation of cumulus masses in the drops of medium for 5 hrs at 37 deg C in the humidified atmosphere of 5 % CO2 in air, the adherent cumulus cells were removed with hyaluronidase (100 unit/ml). The types of parthenogenetic eggs were morphologically c1038lassified into haploid (H), immediate cleavage (IC) and diploid (D) under a inverted microscope. These parthenogenetic eggs were then incubated into the drops of mKRB medium for 96 hrs under the same conditions as a