DC Field | Value | Language |
---|---|---|
dc.contributor.author | Byoung Chul Park | - |
dc.contributor.author | Hyang Sook Lim | - |
dc.contributor.author | Myung Hee Kim | - |
dc.contributor.author | Dae Sil Lee | - |
dc.date.accessioned | 2017-04-19T08:43:45Z | - |
dc.date.available | 2017-04-19T08:43:45Z | - |
dc.date.issued | 1988 | - |
dc.identifier.issn | 0368-4881 | - |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/2994 | - |
dc.description.abstract | Total synthesis of human insulin A and B chain genes were designed for the expression through bacterial system. Eleven individual oligonucleotides corresponding to insulin A and B chains were chemically synthesized by the phosphite triester method on solid support. The enzymatic joining of the oligonucleotides were employed to give the synthetic human insulin A and B chain genes, which ware separately cloned into the bacterium Escherichia coll using plasmid, pUC19. The cloned DNAs were shown to have the correct nucleotide sequence as designed. | - |
dc.publisher | Korea Soc-Assoc-Inst | - |
dc.title | Study of human insulin : I. Synthesis and cloning of genes for human insulin A and B chains | - |
dc.title.alternative | Study of human insulin : I. Synthesis and cloning of genes for human insulin A and B chains | - |
dc.type | Article | - |
dc.citation.title | Korean Biochemical Journal | - |
dc.citation.number | 3 | - |
dc.citation.endPage | 220 | - |
dc.citation.startPage | 214 | - |
dc.citation.volume | 21 | - |
dc.contributor.affiliatedAuthor | Byoung Chul Park | - |
dc.contributor.affiliatedAuthor | Myung Hee Kim | - |
dc.contributor.affiliatedAuthor | Dae Sil Lee | - |
dc.contributor.alternativeName | 박병철 | - |
dc.contributor.alternativeName | 임향숙 | - |
dc.contributor.alternativeName | 김명희 | - |
dc.contributor.alternativeName | 이대실 | - |
dc.identifier.bibliographicCitation | Korean Biochemical Journal, vol. 21, no. 3, pp. 214-220 | - |
dc.description.journalClass | N | - |
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