Study of human insulin : I. Synthesis and cloning of genes for human insulin A and B chains

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dc.contributor.authorByoung Chul Park-
dc.contributor.authorHyang Sook Lim-
dc.contributor.authorMyung Hee Kim-
dc.contributor.authorDae Sil Lee-
dc.date.accessioned2017-04-19T08:43:45Z-
dc.date.available2017-04-19T08:43:45Z-
dc.date.issued1988-
dc.identifier.issn0368-4881-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/2994-
dc.description.abstractTotal synthesis of human insulin A and B chain genes were designed for the expression through bacterial system. Eleven individual oligonucleotides corresponding to insulin A and B chains were chemically synthesized by the phosphite triester method on solid support. The enzymatic joining of the oligonucleotides were employed to give the synthetic human insulin A and B chain genes, which ware separately cloned into the bacterium Escherichia coll using plasmid, pUC19. The cloned DNAs were shown to have the correct nucleotide sequence as designed.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleStudy of human insulin : I. Synthesis and cloning of genes for human insulin A and B chains-
dc.title.alternativeStudy of human insulin : I. Synthesis and cloning of genes for human insulin A and B chains-
dc.typeArticle-
dc.citation.titleKorean Biochemical Journal-
dc.citation.number3-
dc.citation.endPage220-
dc.citation.startPage214-
dc.citation.volume21-
dc.contributor.affiliatedAuthorByoung Chul Park-
dc.contributor.affiliatedAuthorMyung Hee Kim-
dc.contributor.affiliatedAuthorDae Sil Lee-
dc.contributor.alternativeName박병철-
dc.contributor.alternativeName임향숙-
dc.contributor.alternativeName김명희-
dc.contributor.alternativeName이대실-
dc.identifier.bibliographicCitationKorean Biochemical Journal, vol. 21, no. 3, pp. 214-220-
dc.description.journalClassN-
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Critical Diseases Diagnostics Convergence Research Center > 1. Journal Articles
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