Controlled fed-batch cultivation of Escherichia coli mutant for L-tryptophan production = 대장균 변이주의 조절식 유가배양법에 의한 L-트립토판 생산

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Title
Controlled fed-batch cultivation of Escherichia coli mutant for L-tryptophan production = 대장균 변이주의 조절식 유가배양법에 의한 L-트립토판 생산
Author(s)
In Young Lee; Myung Kuk Kim; Yung Hee Kho; Moo-Young Kwak; Hosull Lee; Sun Bok Lee
Bibliographic Citation
Korean Journal of (Applied) Microbiology & Biotechnology, vol. 16, no. 6, pp. 450-456
Publication Year
1988
Abstract
For optimal production of L-tryptophan using a regulatory mutant of Escherichia coli the relationship between product formation and acid production was investigated. Experimental results showed that the production level of L-tryptophan was lowered as the specific acid production rate increased. In order to reduce the amount of acid produced during the fermentation, a controlled fed-batch fermentation was employed. In this fed-batch process, the feed rate of the nutrient feed medium was controlled in relation to the oxygen level in the culture and thus the growth of the cells was regulated in such a way that the oxygen demand of the culture could not exceed the oxygen supply. When E. coli cells were cultivated in a controlled fed-batch mode of fermentor operation, the specific production rate was significantly reduced and L-tryptophan production was increased as as five times that obtained in a conventional fed-batch fermentation.
Keyword
Controlled fed-batchL-tryptophanE. coli
ISSN
0257-2389
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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