High-level expression of the Insecticidal crystal protein gene from Bacillus thuringiensis subsp. kurstaki HD-73 in Escherichia coli

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Title
High-level expression of the Insecticidal crystal protein gene from Bacillus thuringiensis subsp. kurstaki HD-73 in Escherichia coli
Author(s)
So Young Min; Ho Yong Park; Myung Hee Kim; Jeong Il Kim
Bibliographic Citation
Korean Biochemical Journal, vol. 21, no. 4, pp. 372-377
Publication Year
1988
Abstract
A 3.7 kb Ndel fragment (Min et al.,1986,Korean Biochem. J. 19, 363) carrying the, insecticidal crystal protein gene of B. thuringiensis subsp. kurstaki HD-73 was recloned under the tac promoter in pDR540, the E. coli expression vector. The 130-Kdal crystal protein expressed in E. coli was very stable and amounted to about 30% of the total cellular protein under optimal conditions, and most of them are contained in the inclusion body.
ISSN
0368-4881
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
Division of Biomedical Research > Microbiome Convergence Research Center > 1. Journal Articles
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