Covalent and oriented immobilization of antibodies through systematic modification of photoactivatable RNA hybrid aptamers

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dc.contributor.authorChang Hoon Jeon-
dc.contributor.authorTai Hwan Ha-
dc.date.accessioned2022-08-19T16:32:24Z-
dc.date.available2022-08-19T16:32:24Z-
dc.date.issued2022-
dc.identifier.issn1043-1802-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/30186-
dc.description.abstractWe report a covalent and well-oriented strategy of immobilization of antibodies using photoactivatable Fc-binding RNA aptamers (FcBAs). We prepared several types of FcBAs that were further modified with photoaffinity probes (i.e., benzophenone or diazirine), and evaluated the binding capabilities and the photo-crosslinking efficiency of them via pull-down assays and fluorescence analyses, respectively. Initial moderate photo-crosslinking efficiency (Kd ∼ 110 nM) was substantially improved by multivalent association of FcBAs and structural modification of FcBAs. For a conceptual proof, covalent crosslinking of human IgG on FcBA-tethered solid chips was fabricated and investigated by scanometry, which eventually proved real applicability of the present scheme toward immunoassays.-
dc.publisherAmer Chem Soc-
dc.titleCovalent and oriented immobilization of antibodies through systematic modification of photoactivatable RNA hybrid aptamers-
dc.title.alternativeCovalent and oriented immobilization of antibodies through systematic modification of photoactivatable RNA hybrid aptamers-
dc.typeArticle-
dc.citation.titleBioconjugate Chemistry-
dc.citation.number8-
dc.citation.endPage1535-
dc.citation.startPage1527-
dc.citation.volume33-
dc.contributor.affiliatedAuthorChang Hoon Jeon-
dc.contributor.affiliatedAuthorTai Hwan Ha-
dc.contributor.alternativeName전창훈-
dc.contributor.alternativeName하태환-
dc.identifier.bibliographicCitationBioconjugate Chemistry, vol. 33, no. 8, pp. 1527-1535-
dc.identifier.doi10.1021/acs.bioconjchem.2c00274-
dc.description.journalClassY-
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Division of Bio Technology Innovation > Core Research Facility & Analysis Center > 1. Journal Articles
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