DC Field | Value | Language |
---|---|---|
dc.contributor.author | Chang Hoon Jeon | - |
dc.contributor.author | Tai Hwan Ha | - |
dc.date.accessioned | 2022-08-19T16:32:24Z | - |
dc.date.available | 2022-08-19T16:32:24Z | - |
dc.date.issued | 2022 | - |
dc.identifier.issn | 1043-1802 | - |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/30186 | - |
dc.description.abstract | We report a covalent and well-oriented strategy of immobilization of antibodies using photoactivatable Fc-binding RNA aptamers (FcBAs). We prepared several types of FcBAs that were further modified with photoaffinity probes (i.e., benzophenone or diazirine), and evaluated the binding capabilities and the photo-crosslinking efficiency of them via pull-down assays and fluorescence analyses, respectively. Initial moderate photo-crosslinking efficiency (Kd ∼ 110 nM) was substantially improved by multivalent association of FcBAs and structural modification of FcBAs. For a conceptual proof, covalent crosslinking of human IgG on FcBA-tethered solid chips was fabricated and investigated by scanometry, which eventually proved real applicability of the present scheme toward immunoassays. | - |
dc.publisher | Amer Chem Soc | - |
dc.title | Covalent and oriented immobilization of antibodies through systematic modification of photoactivatable RNA hybrid aptamers | - |
dc.title.alternative | Covalent and oriented immobilization of antibodies through systematic modification of photoactivatable RNA hybrid aptamers | - |
dc.type | Article | - |
dc.citation.title | Bioconjugate Chemistry | - |
dc.citation.number | 8 | - |
dc.citation.endPage | 1535 | - |
dc.citation.startPage | 1527 | - |
dc.citation.volume | 33 | - |
dc.contributor.affiliatedAuthor | Chang Hoon Jeon | - |
dc.contributor.affiliatedAuthor | Tai Hwan Ha | - |
dc.contributor.alternativeName | 전창훈 | - |
dc.contributor.alternativeName | 하태환 | - |
dc.identifier.bibliographicCitation | Bioconjugate Chemistry, vol. 33, no. 8, pp. 1527-1535 | - |
dc.identifier.doi | 10.1021/acs.bioconjchem.2c00274 | - |
dc.description.journalClass | Y | - |
There are no files associated with this item.
Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.