Generation of monoclonal antibody against hepatitis B virus (HBV) Pre-S2 peptide

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Title
Generation of monoclonal antibody against hepatitis B virus (HBV) Pre-S2 peptide
Author(s)
Young Ik Lee; Suhn Young Im; Young Hoon Kim; Mi Seon Yoon; Kyung Soo Hahm; Moon Hi Han
Bibliographic Citation
Korean Biochemical Journal, vol. 22, no. 3, pp. 355-360
Publication Year
1989
Abstract
Monoclonal antibody against HBV pre-S2 peptide was generated. Six clones among 70 hybridomas which were survived in HAT media produced anti pre-S2 antibody. All six clones has been identified to produce IgM-type monoclonal antibody which has kappa light chain after ELISA subtyping analysis. After purification of IgM monoclonal antibody, heavy and light chain of immunoglobulin has been identified whose molecular weights are 79KD and 26.5KD, respectively. These pre-S2 antibodies were confirmed using immunoblotting techniques. IgM type pre-S2 monoclonal antibody were bound to 55 amino acid pre-S2 antigen whose M.W. is 55KD.
ISSN
0368-4881
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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