Purification and characterization of ice nucleating protein from Pseudomonas syringae

Cited 0 time in scopus
Metadata Downloads
Title
Purification and characterization of ice nucleating protein from Pseudomonas syringae
Author(s)
Jeongha Kim; Soon-Ho Park; Moon Hi Han; Kyung Soo Hahm
Bibliographic Citation
Korean Biochemical Journal, vol. 22, no. 1, pp. 73-77
Publication Year
1989
Abstract
The protein responsible for ice-nucleation activity by Pseudomonas syringae AL (KCTC 1832) has been purified. The protein was purified from the outer membrane fraction of the cell by methods including extraction with non-denaturing detergents octyl-分-D-thio-glucopyranoside, gel filtration chromatography on Sephacryl S-400 and gel electroelution. The molecular weight of the ice-nucleating protein is estimated to be about 150,000 dalton by SDS-PAGE. Ice-nucleation activity was measured by the micro-pipette method. Ice-nuclea-tion activity of the purified protein was about -8?9 °C, whereas the freezing point of supercooled water is -17 °C.
ISSN
0368-4881
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.