The proliferative and multipotent epidermal progenitor cells for human skin reconstruction in vitro and in vivo

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Title
The proliferative and multipotent epidermal progenitor cells for human skin reconstruction in vitro and in vivo
Author(s)
Jung Hwa Lim; Dae Hun Kim; Kyung Hee NohCho-Rok JungHyun Mi Kang
Bibliographic Citation
Cell Proliferation, vol. 55, no. 9, pp. e13284-e13284
Publication Year
2022
Abstract
Objectives: The skin exhibits tremendous regenerative potential, as different types of progenitor and stem cells regulate skin homeostasis and damage. However, in vitro primary keratinocytes present with several drawbacks, such as high donor variability, short lifespan, and limited donor tissue availability. Therefore, more stable primary keratinocytes are needed to generate multiple uniform in vitro and in vivo skin models. Results: We identified epidermal progenitor cells from primary keratinocytes using Integrin beta 1 (ITGB1) an epidermal stem cell marker markedly decreased after senescence in vitro. Epidermal progenitor cells exhibited unlimited proliferation and the potential for multipotent differentiation capacity. Moreover, they could completely differentiate to form an organotypic skin model including conversed mesenchymal cells in the dermis and could mimic the morphologic and biochemical processes of human epidermis. We also discovered that proliferation and the multipotent differentiation capacity of these cells relied on ITGB1 expression. Eventually, we examined the in vitro and in vivo wound healing capacity of these epidermal progenitor cells. Conclusions: Overall, the findings suggest that these stable and reproducible cells can differentiate into multiple lineages, including human skin models. They are a potentially powerful tool for studying skin regeneration, skin diseases, and are an alternative for in vivo experiments.
ISSN
0960-7722
Publisher
Wiley
DOI
http://dx.doi.org/10.1111/cpr.13284
Type
Article
Appears in Collections:
Division of Research on National Challenges > Stem Cell Convergenece Research Center > 1. Journal Articles
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