A simplified procedure for C-reactive protein purification

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dc.contributor.authorHee Gu Lee-
dc.contributor.authorYong Ho Kim-
dc.contributor.authorHong Soo Lee-
dc.contributor.authorMyung Ja Choi-
dc.contributor.authorIn Seong Choe-
dc.contributor.authorTai Wha Chung-
dc.date.accessioned2017-04-19T08:43:50Z-
dc.date.available2017-04-19T08:43:50Z-
dc.date.issued1989-
dc.identifier.issn0368-4881-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/3035-
dc.description.abstractA simplified procedure for obtaining high purity and high yield of C-reactive protein (CRP) was established. The CRP from ascitic and pleural fluid was purified using calcium dependent affinity chromatography of CNBr-activated Sepharose-4B covalently coupled to p-diazonium phenylphosphorylcholine (DPPC) and hydroxylapatite chromatography. The active fractions of CRP during the purification procedure were determined by EIA using pneumococcal C-polysaccharide. The final recovery of CRP was 67% of the initial protein amount as quantitated by single radial immunodiffusion (SRID), and the purification fold was 3,600. The purified CRP was shown to be homogeneous by electrophoresis and immunoelectrophoresis. The molecular weight of CRP was approximately 118’KD as determined by Sephacryl S-200 gel filtration and the molecular weight of a subunit was approximately 23.6 KD by SDS-polyacrylamide gel electrophoresis. The isoelectric point of CRP was found to range about 4.82.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleA simplified procedure for C-reactive protein purification-
dc.title.alternativeA simplified procedure for C-reactive protein purification-
dc.typeArticle-
dc.citation.titleKorean Biochemical Journal-
dc.citation.number4-
dc.citation.endPage454-
dc.citation.startPage448-
dc.citation.volume22-
dc.contributor.affiliatedAuthorHee Gu Lee-
dc.contributor.affiliatedAuthorHong Soo Lee-
dc.contributor.affiliatedAuthorIn Seong Choe-
dc.contributor.affiliatedAuthorTai Wha Chung-
dc.contributor.alternativeName이희구-
dc.contributor.alternativeName김용호-
dc.contributor.alternativeName이홍수-
dc.contributor.alternativeName최명자-
dc.contributor.alternativeName최인성-
dc.contributor.alternativeName정태화-
dc.identifier.bibliographicCitationKorean Biochemical Journal, vol. 22, no. 4, pp. 448-454-
dc.description.journalClassN-
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Division of Biomedical Research > Immunotherapy Research Center > 1. Journal Articles
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