miR-302 suppresses the proliferation, migration, and invasion of breast cancer cells by downregulating ATAD2

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Title
miR-302 suppresses the proliferation, migration, and invasion of breast cancer cells by downregulating ATAD2
Author(s)
Yo Sep Hwang; Eun Sun Park; Byung Moo Oh; T G Uhm; Suk Ran YoonJong Lyul ParkHee Jun ChoHee Gu Lee
Bibliographic Citation
Cancers, vol. 14, no. 18, pp. 4345-4345
Publication Year
2022
Abstract
Breast cancer is the most common malignant tumor in women. The ATPase family AAA domain-containing protein 2 (ATAD2) contains an ATPase domain and a bromodomain, and is abnormally expressed in various human cancers, including breast cancer. However, the molecular mechanisms underlying the regulation of ATAD2 expression in breast cancer remain unclear. This study aimed to investigate the expression and function of ATAD2 in breast cancer. We found that ATAD2 was highly expressed in human breast cancer tissues and cell lines. ATAD2 depletion via RNA interference inhibited the proliferation, migration, and invasive ability of the SKBR3 and T47D breast cancer cell lines. Furthermore, Western blot analysis and luciferase assay results revealed that ATAD2 is a putative target of miR-302. Transfection with miR-302 mimics markedly reduced cell migration and invasion. These inhibitory effects of miR-302 were restored by ATAD2 overexpression. Moreover, miR-302 overexpression in SKBR3 and T47D cells suppressed tumor growth in the xenograft mouse model. However, ATAD2 overexpression rescued the decreased tumor growth seen after miR-302 overexpression. Our findings indicate that miR-302 plays a prominent role in inhibiting the cancer cell behavior associated with tumor progression by targeting ATAD2, and could thus be a valuable target for breast cancer therapy.
Keyword
ATAD2miR-302Breast cancerProliferationInvasionTumor growth
ISSN
2072-6694
Publisher
MDPI
DOI
http://dx.doi.org/10.3390/cancers14184345
Type
Article
Appears in Collections:
Division of Biomedical Research > Immunotherapy Research Center > 1. Journal Articles
Aging Convergence Research Center > 1. Journal Articles
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