A sensitive immunodetection assay using antibodies specific to staphylococcal enterotoxin B produced by baculovirus expression

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Title
A sensitive immunodetection assay using antibodies specific to staphylococcal enterotoxin B produced by baculovirus expression
Author(s)
Ju Hong Jang; Sungsik Kim; Seul Gi Kim; Jaemin Lee; Dong Gwang Lee; Jieun Jang; Y S Jeong; D H Song; Jeong Ki Min; Jong Gil ParkMoo-Seung LeeBaek Soo Han; J S Son; Jangwook LeeNam-Kyung Lee
Bibliographic Citation
Biosensors, vol. 12, no. 10, pp. 787-787
Publication Year
2022
Abstract
Staphylococcal enterotoxin B (SEB) is a potent bacterial toxin that causes inflammatory stimulation and toxic shock, thus it is necessary to detect SEB in food and environmental samples. Here, we developed a sensitive immunodetection system using monoclonal antibodies (mAbs). Our study is the first to employ a baculovirus expression vector system (BEVS) to produce recombinant wild-type SEB. BEVS facilitated high-quantity and pure SEB production from suspension-cultured insect cells, and the SEB produced was characterized by mass spectrometry analysis. The SEB was stable at 4 °C for at least 2 years, maintaining its purity, and was further utilized for mouse immunization to generate mAbs. An optimal pair of mAbs non-competitive to SEB was selected for sandwich enzyme-linked immunosorbent assay-based immunodetection. The limit of detection of the immunodetection method was 0.38 ng/mL. Moreover, it displayed higher sensitivity in detecting SEB than commercially available immunodetection kits and retained detectability in various matrices and S. aureus culture supernatants. Thus, the results indicate that BEVS is useful for producing pure recombinant SEB with its natural immunogenic property in high yield, and that the developed immunodetection assay is reliable and sensitive for routine identification of SEB in various samples, including foods.
Keyword
Staphylococcal enterotoxin BBaculovirus expression vector systemMonoclonal antibodiesImmunodetectionSandwich ELISA
ISSN
2079-6374
Publisher
MDPI
DOI
http://dx.doi.org/10.3390/bios12100787
Type
Article
Appears in Collections:
Division of Biomedical Research > Biotherapeutics Translational Research Center > 1. Journal Articles
Division of Research on National Challenges > Environmental diseases research center > 1. Journal Articles
Division of Research on National Challenges > Biodefense Research Center > 1. Journal Articles
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