Enhanced mitochondrial DNA editing in mice using nuclear-exported TALE-linked deaminases and nucleases

Cited 13 time in scopus
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Title
Enhanced mitochondrial DNA editing in mice using nuclear-exported TALE-linked deaminases and nucleases
Author(s)
S Lee; Hyunji Lee; G Baek; E Namgung; J M Park; Sanghun Kim; Seongho Hong; J S Kim
Bibliographic Citation
Genome Biology, vol. 23, pp. 211-211
Publication Year
2022
Abstract
We present two methods for enhancing the efficiency of mitochondrial DNA (mtDNA) editing in mice with DddA-derived cytosine base editors (DdCBEs). First, we fused DdCBEs to a nuclear export signal (DdCBE-NES) to avoid off-target C-to-T conversions in the nuclear genome and improve editing efficiency in mtDNA. Second, mtDNA-targeted TALENs (mitoTALENs) are co-injected into mouse embryos to cleave unedited mtDNA. We generated a mouse model with the m.G12918A mutation in the MT-ND5 gene, associated with mitochondrial genetic disorders in humans. The mutant mice show hunched appearances, damaged mitochondria in kidney and brown adipose tissues, and hippocampal atrophy, resulting in premature death.
Keyword
DdCBENESmitoTALENmtDNAMitochondrial DNA editing
ISSN
1474-760X
Publisher
Springer-BMC
DOI
http://dx.doi.org/10.1186/s13059-022-02782-z
Type
Article
Appears in Collections:
Ochang Branch Institute > Division of National Bio-Infrastructure > Laboratory Animal Resource & Research Center > 1. Journal Articles
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