Cited 13 time in
- Title
- Enhanced mitochondrial DNA editing in mice using nuclear-exported TALE-linked deaminases and nucleases
- Author(s)
- S Lee; Hyunji Lee; G Baek; E Namgung; J M Park; Sanghun Kim; Seongho Hong; J S Kim
- Bibliographic Citation
- Genome Biology, vol. 23, pp. 211-211
- Publication Year
- 2022
- Abstract
- We present two methods for enhancing the efficiency of mitochondrial DNA (mtDNA) editing in mice with DddA-derived cytosine base editors (DdCBEs). First, we fused DdCBEs to a nuclear export signal (DdCBE-NES) to avoid off-target C-to-T conversions in the nuclear genome and improve editing efficiency in mtDNA. Second, mtDNA-targeted TALENs (mitoTALENs) are co-injected into mouse embryos to cleave unedited mtDNA. We generated a mouse model with the m.G12918A mutation in the MT-ND5 gene, associated with mitochondrial genetic disorders in humans. The mutant mice show hunched appearances, damaged mitochondria in kidney and brown adipose tissues, and hippocampal atrophy, resulting in premature death.
- Keyword
- DdCBENESmitoTALENmtDNAMitochondrial DNA editing
- ISSN
- 1474-760X
- Publisher
- Springer-BMC
- DOI
- http://dx.doi.org/10.1186/s13059-022-02782-z
- Type
- Article
- Appears in Collections:
- Ochang Branch Institute > Division of National Bio-Infrastructure > Laboratory Animal Resource & Research Center > 1. Journal Articles
- Files in This Item:
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