Development of a model system for artificial seed production : II. a dry type of carrot(Daucus carota L.) artificial seeds = 인공종자 생산의 모델 시스템 개발 : Ⅱ. 당근의 건조형 인공종자

Abstract

Somatic embryos from cell suspension cultures of carrot were singly encapsulated with alginic acid to be used as artificial seeds. Most of the freshly encapsulated embryos germinated while stored at 4°C: a hydrated capsule per se retained enough water for the encapsulated embryo to germinate. However, germination was effectively blocked, when hydrated capsules were subjected to a proper air-blowing dehydration to the level of over 80% water loss. the initial fresh weight - the weight afterof alginate capsules dehydration( % water loss x 100 )the initial fresh weight of alginate capsules. Ninety-seven% of the capsules, dehydrated to the level of 88% water loss, were capable of germination upon sub明quent rehydration. Drying rate in the range of 0.1-0.4 g (water/hi did not influence the subsequent germinability of dehydrated capsules. As the length of embryos before encapsulation increased up to 3 mm, the germination rate of dehydrated capsules following rehydration increased. Pretreatment of embryos with abscisic acid (ABA) for 10 days prior to encapsulation enhanced the germination rate of capsules which were dehydrated to the level of 95% water loss following rehydration: as the concentration of ABA increased to 0.13 mg/C, the germination rate also increased by up to 31%. Sixty-eight% of the capsules, dehydrated to the level of 92% water loss, germinated after 10 days of storage at 4°C upon subsequent iehydration. These results indicate that the dehydrated capsule containing a somatic embryo has greater analogy to the botanical seed in the form and function than the hydrated capsule.