Control of In vitro flowering of ginseng(Panax ginseng C.A. Meyer) by growth regulators = 생장조절제에 의한 인삼(Panax ginseng C.A. Meyer)의 기내 화아형성 조절

Abstract

Ginseng zygotic embryos, seedlings, and excised cotyledonary nodes were cultured on Murashige and Skoog's (MS) medium, supplemented with 6-benzyladenine (BA) and gibberellic acid (GA3) to induce flower buds. As the concentration of nitrogen compounds in MS medium was reduced to half of its strength, the flowering frequency of zygotic embryos increased up to 90 %. The optimum concentration of sucrose in the medium for flowering of seedlings was 30-60 g/l. In all cases flower buds were formed on elongated axillary branches from the cotyledonary node, while the apices remained vegetative. When zygotic embryos and excised cotyledonary nodes were cultured on the medium, supplemented with all possible combinations of BA, GA3, and abscisic acid (ABA) of 5 micro M each, flowering was induced on the medium with either BA, BA + GA3, or BA + GA3 + ABA. However, when seedlings were employed, flowering was induced on the medium with either BA, BA + GA3, BA + ABA, or BA + GA3 + ABA. In addition, inclusion of 5 micro M indole-3-acetic acid (IAA) in the above combinations did not affect flowering. These results suggest that cytokinins, gibberellins, and inhibitors play primary, permissive, and preventive roles, respectively, in the induction of flowering of ginseng