Callus formation from suspension culture-derived protoplasts of sweet potato(Ipomoea batatas) = 고구마(Ipomoea batatas)의 현탁배양 세포의 원형질체 배양에 의한 캘러스 형성

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Title
Callus formation from suspension culture-derived protoplasts of sweet potato(Ipomoea batatas) = 고구마(Ipomoea batatas)의 현탁배양 세포의 원형질체 배양에 의한 캘러스 형성
Author(s)
Jang Ryol Liu; D J Cantliffe
Bibliographic Citation
Korean Journal of Botany, vol. 32, no. 4, pp. 247-253
Publication Year
1989
Abstract
Protoplasts were enzymatically isolated from suspension cultures of sweet potato. High yields of single protoplasts were produced from nonembryogenic cell aggregates. However, most protoplasts obtained from embryogenic cell clumps were spontaneously fused during enzyme treatment; a small portion of them remained single. Upon transfer to Murashige and Skoog's(MS) liquid medium supplemented with 0.1 mg/l 6-benzyladenine (BA) and 1 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D), protoplasts from nonembryogenic cell aggregates sustained cell divisions to form callus. Upon subculture onto MS media with 0.2 mg/l 2,4-D or without growth regulators, the callus did not give rise to any organs. On the other hand, first cell division of single protoplasts from embryogenic cell clumps was sporadically observed
ISSN
I000-0012
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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