Intermediate cells of in vitro cellular reprogramming and in vivo tissue regeneration require desmoplakin

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Title
Intermediate cells of in vitro cellular reprogramming and in vivo tissue regeneration require desmoplakin
Author(s)
Jeongmin Ha; B S Kim; Byungkuk Min; Juhyeon Nam; Jae-Geun Lee; Minhyung Lee; Byoung-Ha Yoon; Y H Choi; I Im; Jung Sun Park; H Choi; Areum BaekSang Mi ChoMi Ok LeeKi Hoan Nam; J Y Mun; Mirang KimSeon-Young KimMi-Young SonYong-Kook KangJeong Soo Lee; J K Kim; Janghwan Kim
Bibliographic Citation
Science Advances, vol. 8, no. 43, pp. eabk1239-eabk1239
Publication Year
2022
Abstract
Amphibians and fish show considerable regeneration potential via dedifferentiation of somatic cells into blastemal cells. In terms of dedifferentiation, in vitro cellular reprogramming has been proposed to share common processes with in vivo tissue regeneration, although the details are elusive. Here, we identified the cytoskeletal linker protein desmoplakin (Dsp) as a common factor mediating both reprogramming and regeneration. Our analysis revealed that Dsp expression is elevated in distinct intermediate cells during in vitro reprogramming. Knockdown of Dsp impedes in vitro reprogramming into induced pluripotent stem cells and induced neural stem/progenitor cells as well as in vivo regeneration of zebrafish fins. Notably, reduced Dsp expression impairs formation of the intermediate cells during cellular reprogramming and tissue regeneration. These findings suggest that there is a Dsp-mediated evolutionary link between cellular reprogramming in mammals and tissue regeneration in lower vertebrates and that the intermediate cells may provide alternative approaches for mammalian regenerative therapy.
ISSN
2375-2548
Publisher
Amer Assoc Advancement Science
DOI
http://dx.doi.org/10.1126/sciadv.abk1239
Type
Article
Appears in Collections:
Aging Convergence Research Center > 1. Journal Articles
Division of Research on National Challenges > Stem Cell Convergenece Research Center > 1. Journal Articles
Ochang Branch Institute > Division of National Bio-Infrastructure > Laboratory Animal Resource & Research Center > 1. Journal Articles
Division of Biomedical Research > Microbiome Convergence Research Center > 1. Journal Articles
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