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- A highly efficient and versatile genetic engineering toolkit for a methanotroph-based biorefinery
- Jiyeong Jeong; Tae Hyun Kim; Nulee Jang; Minji Ko; Seong Keun Kim; Ji In Baek; Georgii Emelianov; Eugene Rha; Kil Koang Kwon; Haseong Kim; E Y Lee; Dae Hee Lee; Hyewon Lee; Seung Goo Lee
- Bibliographic Citation
- Chemical Engineering Journal, vol. 453, pp. 139911-139911
- Publication Year
- Methanotrophs are promising and sustainable cell factory platforms owing to their ability to convert the most potent greenhouse gas, methane to valuable bioproducts. Genetic engineering toolkits for methanotrophs are extremely limited. Here, we present a phenol-inducible promoter for the high-level expression of exogenous genes in methanotrophs. The phenol-inducible gene expression system showed high dose-dependency and homogeneity in methanotrophs. Using the phenol-inducible CRISPR-base editor (BE), we developed a highly efficient methanotroph genome editing system. The CRISPR-BE system efficiently introduced an early stop codon into the target gene, enabling one-step markerless genome editing in Methylococcus capsulatus Bath. We adopted this simple and efficient genome editing tool to produce mevalonate in the engineered M. capsulatus Bath. The native phosphoketolase pathway was reinforced in M. capsulatus Bath to increase the carbon flux via acetyl-CoA towards mevalonate. This engineered M. capsulatus Bath produced the maximum concentration of 2,090 mg/L mevalonate from methane, which is the highest amount of synthetic biochemicals produced from methane in methanotrophs. Here we present not only an efficient addition to the genetic engineering toolkit for methanotrophs but also a useful platform for the development of a methanotroph cell factory.
- MethaneMethanotrophPhenol-inducible promoterCRISPR-base editorPhosphoketolaseMevalonate
- Appears in Collections:
- Synthetic Biology and Bioengineering Research Institute > Synthetic Biology Research Center > 1. Journal Articles
Synthetic Biology and Bioengineering Research Institute > 1. Journal Articles
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