Fructose production from Jerusalem artichoke by inulinase immobilized on chitin

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Title
Fructose production from Jerusalem artichoke by inulinase immobilized on chitin
Author(s)
Chul Ho Kim; Sang Ki Rhee
Bibliographic Citation
Biotechnology Letters, vol. 11, no. 3, pp. 201-206
Publication Year
1989
Abstract
Inulinase from Aspergillus ficuum was immobilized by cross-linking with glutaraldehyde on chitin. Batch and continuous production of fructose from Jerusalem artichoke tuber was studied using this immobililized inulinase. In a batch reactor, the extent of hydrolysis attained 90% (D-fructose/D-glucose :86/14) in 10h and 77.5g/L of D-fructose was produced from the Jerusalem artichoke tuber juice. In a continuous packed bed column reactor, the maximum volumetric productivity of 61 g/L, h was obtained at residence time of 0.9h and conversion yield of 55%. At a fixed residence time of 2.6 h and 40° C, this could be operated for over two weeks with only a slight loss of activity (4.8%).
ISSN
0141-5492
Publisher
Springer
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
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