Cloning and expression of human alpha1-antitrypsin cDNA in yeast Saccharomyces cerevisiae = 효모에서 인체 alpha1-antitrypsin cDNA의 클로닝 및 발현

Abstract

Human a 1-antitrypsin cDNA was expressed in yeast Saccharomyces cerevisiae under the regulation of GAL10-CYC1 hybrid promoter. A suitable Gal+ host strain was constructed by tetrad analysis of a diploid from a cross of two Gal' strains, each affected in a different galactose utilizing gene. The resulting strain KY8 was transformed with the expression plasmid pGAT7 and the synthesis of a 1-antitrypsin was induced in galactose medium. Production of al-antitrypsin in recombinant yeast cells was confirmed by an elastase inhibitory assay and an immunoblotting method. The molecular weight of recombinant al-antitryp-sin synthesized in yeast was 44 kDa which was expected from the full length cDNA sequence of the crl-antitrypsin structural gene.