In vitro anti-inflammatory effects of Symplocos sumuntia Buch.-Ham. ex D. Don extract via blockage of the NF-κB/JNK signaling pathways in LPS-activated microglial cells

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Title
In vitro anti-inflammatory effects of Symplocos sumuntia Buch.-Ham. ex D. Don extract via blockage of the NF-κB/JNK signaling pathways in LPS-activated microglial cells
Author(s)
J S Lim; Jaehoon Bae; S Lee; D Y Lee; L Yao; N Cho; T T Bach; Narae Yun; Su-Jin Park; Y C Cho
Bibliographic Citation
Plants-Basel, vol. 11, no. 22, pp. 3095-3095
Publication Year
2022
Abstract
Symplocos sumuntia Buch.-Ham. ex D. Don (S. sumuntia) is a traditional medicinal herb used in Asia to treat various pathologies, including cough, stomachache, tonsillitis, hypertension, and hyperlipidemia. Although the anti-inflammatory activity of S. sumuntia has been reported, little is known about its anti-inflammatory activity and molecular mechanisms in microglial cells. Therefore, we investigated the inhibitory effects of S. sumuntia methanol extract (SSME) on the inflammatory responses in lipopolysaccharide (LPS)-treated BV2 cells. The SSME significantly inhibited the LPS-stimulated inducible nitric oxide synthase and cyclooxygenase-2 expression, as well as the production of nitric oxide (NO), a proinflammatory mediator. The production of proinflammatory cytokines, including interleukin (IL)-6, tumor necrosis factor-α, and IL-1β, was suppressed by the SSME in the LPS-induced BV2 cells. The mechanism underlying the anti-inflammatory effects of SSME involves the suppression of the LPS-stimulated phosphorylation of mitogen-activated protein kinases (MAPKs) such as JNK. Moreover, we showed that the LPS-stimulated nuclear translocation of the nuclear factor-κB (NF-κB)/p65 protein, followed by IκB degradation, was decreased by the SSME treatment. Collectively, these results showed that the SSME induced anti-inflammatory effects via the suppression of the MAPK signaling pathways, accompanied by changes in the NF-κB translocation into the nucleus. Therefore, SSME may be employed as a potential therapeutic candidate for various inflammatory diseases.
Keyword
Symplocos sumuntia Buch.-Ham. ex D. DonAnti-inflammatory activityMicroglial cellsMAPK signaling pathwaysNF-kB translocation
ISSN
2223-7747
Publisher
MDPI
DOI
http://dx.doi.org/10.3390/plants11223095
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Functional Biomaterial Research Center > 1. Journal Articles
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