Rapid and accurate on-site immunodiagnostics of highly contagious severe acute respiratory syndrome coronavirus 2 using portable surface-enhanced raman scattering-lateral flow assay reader
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- Rapid and accurate on-site immunodiagnostics of highly contagious severe acute respiratory syndrome coronavirus 2 using portable surface-enhanced raman scattering-lateral flow assay reader
- Y Joung; K Kim; S Lee; B S Chun; S Lee; J Hwang; S Choi; Taejoon Kang; M K Lee; L Chen; J Choo
- Bibliographic Citation
- ACS Sensors, vol. 7, no. 11, pp. 3470-3480
- Publication Year
- In early 2022, the number of people infected with the highly contagious mutant severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), called Omicron, was increasing worldwide. Therefore, several countries approved the lateral flow assay (LFA) strip as a diagnostic method for confirming SARS-CoV-2 instead of reverse transcription-polymerase chain reaction (RT-PCR), which takes a long time to generate the results. However, owing to the limitation of detection sensitivity, commercial LFA strips have high false-negative diagnosis rates for patients with low virus concentrations. Therefore, in this study, we developed a portable surface-enhanced Raman scattering (SERS)-LFA reader based on localized surface plasmon effects to solve the sensitivity problem of the commercial LFA strip. We tested 54 clinical samples using this portable SERS-LFA reader, which generated 49 positive and 5 negative results. Out of the 49 positive results, SERS-LFA classified only 2 as false negative, while the commercial LFA classified 21 as false negative. This confirmed that the false-negative rate had significantly improved compared to that of commercial LFA strips. We believe that the proposed SERS-LFA system can be utilized as a point-of-care diagnostic system to quickly and accurately determine a virus infection that could spread significantly within a short period.
- Surface-enhanced Raman scatteringSERS-LFASARS-CoV-2Portable Raman readerImmunoassay
- Amer Chem Soc
- Appears in Collections:
- Division of Research on National Challenges > Bionanotechnology Research Center > 1. Journal Articles
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