Submergence promotes auxin-induced callus formation through ethylene-mediated post-transcriptional control of auxin receptors

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dc.contributor.authorSeung Yong Shin-
dc.contributor.authorY Choi-
dc.contributor.authorS G Kim-
dc.contributor.authorSu-Jin Park-
dc.contributor.authorJi-Sun Park-
dc.contributor.authorKi Beom Moon-
dc.contributor.authorHyun-Soon Kim-
dc.contributor.authorJae Heung Jeon-
dc.contributor.authorHye Sun Cho-
dc.contributor.authorHyo Jun Lee-
dc.date.accessioned2022-12-06T16:32:43Z-
dc.date.available2022-12-06T16:32:43Z-
dc.date.issued2022-
dc.identifier.issn1674-2052-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/30682-
dc.description.abstractPlant cells in damaged tissue can be reprogrammed to acquire pluripotency and induce callus formation. However, in the aboveground organs of many species, somatic cells that are distal to the wound site become less sensitive to auxin-induced callus formation, suggesting the existence of repressive regulatory mechanisms that are largely unknown. Here we reveal that submergence-induced ethylene signals promote callus formation by releasing post-transcriptional silencing of auxin receptor transcripts in non-wounded regions. We determined that short-term submergence of intact seedlings induces auxin-mediated cell dedifferentiation across the entirety of Arabidopsis thaliana explants. The constitutive triple response 1-1 (ctr1-1) mutation induced callus formation in explants without submergence, suggesting that ethylene facilitates cell dedifferentiation. We show that ETHYLENE-INSENSITIVE 2 (EIN2) post-transcriptionally regulates the abundance of transcripts for auxin receptor genes by facilitating microRNA393 degradation. Submergence-induced calli in non-wounded regions were suitable for shoot regeneration, similar to those near the wound site. We also observed submergence-promoted callus formation in Chinese cabbage (Brassica rapa), indicating that this may be a conserved mechanism in other species. Our study identifies previously unknown regulatory mechanisms by which ethylene promotes cell dedifferentiation and provides a new approach for boosting callus induction efficiency in shoot explants.-
dc.publisherElsevier-Cell Press-
dc.titleSubmergence promotes auxin-induced callus formation through ethylene-mediated post-transcriptional control of auxin receptors-
dc.title.alternativeSubmergence promotes auxin-induced callus formation through ethylene-mediated post-transcriptional control of auxin receptors-
dc.typeArticle-
dc.citation.titleMolecular Plant-
dc.citation.number12-
dc.citation.endPage1961-
dc.citation.startPage1947-
dc.citation.volume15-
dc.contributor.affiliatedAuthorSeung Yong Shin-
dc.contributor.affiliatedAuthorSu-Jin Park-
dc.contributor.affiliatedAuthorJi-Sun Park-
dc.contributor.affiliatedAuthorKi Beom Moon-
dc.contributor.affiliatedAuthorHyun-Soon Kim-
dc.contributor.affiliatedAuthorJae Heung Jeon-
dc.contributor.affiliatedAuthorHye Sun Cho-
dc.contributor.affiliatedAuthorHyo Jun Lee-
dc.contributor.alternativeName신승용-
dc.contributor.alternativeName최유리-
dc.contributor.alternativeName김상규-
dc.contributor.alternativeName박수진-
dc.contributor.alternativeName박지선-
dc.contributor.alternativeName문기범-
dc.contributor.alternativeName김현순-
dc.contributor.alternativeName전재흥-
dc.contributor.alternativeName조혜선-
dc.contributor.alternativeName이효준-
dc.identifier.bibliographicCitationMolecular Plant, vol. 15, no. 12, pp. 1947-1961-
dc.identifier.doi10.1016/j.molp.2022.11.001-
dc.subject.keywordCell dedifferentiation-
dc.subject.keywordAuxin signaling-
dc.subject.keywordEthylene-
dc.subject.keywordCallus formation-
dc.subject.keywordEIN2-
dc.subject.localCell dedifferentiation-
dc.subject.localAuxin signaling-
dc.subject.localEthylene-
dc.subject.localethylene-
dc.subject.localCallus formation-
dc.subject.localEIN2-
dc.description.journalClassY-
Appears in Collections:
Division of Research on National Challenges > Plant Systems Engineering Research > 1. Journal Articles
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