Cloning and expression of human lipocortin-1 cDNA in E. coli = 인간 리포코틴-1 유전자의 클로닝 및 대장균에서의 발현
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| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | Kwahng Rae Huh | - |
| dc.contributor.author | Soon Hee Park | - |
| dc.contributor.author | Sung Mang Kang | - |
| dc.contributor.author | In Sung Song | - |
| dc.contributor.author | Hay Young Lee | - |
| dc.contributor.author | Doe Sun Na | - |
| dc.date.accessioned | 2017-04-19T08:43:54Z | - |
| dc.date.available | 2017-04-19T08:43:54Z | - |
| dc.date.issued | 1990 | - |
| dc.identifier.issn | 0368-4881 | - |
| dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/3068 | - |
| dc.description.abstract | A cDNA clone coding for lipocortin-1 was isolated from the cDNA library of human placental mRNA. Plasmid pHTl was constructed by inserting the coding sequence into plasmid pKK233-2, a universal expression vector containing trc promoter. Lipocortin-1 was expressed constitutively or by induction with IPTG in E. coli strain C600 or JM109 harboring pHTl. The activity of purified lipocortin-1 was demonstrated by the in vitro phospholipase A2 inhibitory activity. | - |
| dc.publisher | Korea Soc-Assoc-Inst | - |
| dc.title | Cloning and expression of human lipocortin-1 cDNA in E. coli = 인간 리포코틴-1 유전자의 클로닝 및 대장균에서의 발현 | - |
| dc.title.alternative | Cloning and expression of human lipocortin-1 cDNA in E. coli | - |
| dc.type | Article | - |
| dc.citation.title | Korean Biochemical Journal | - |
| dc.citation.number | 4 | - |
| dc.citation.endPage | 464 | - |
| dc.citation.startPage | 459 | - |
| dc.citation.volume | 23 | - |
| dc.contributor.affiliatedAuthor | Kwahng Rae Huh | - |
| dc.contributor.affiliatedAuthor | Soon Hee Park | - |
| dc.contributor.alternativeName | 허광래 | - |
| dc.contributor.alternativeName | 박순희 | - |
| dc.contributor.alternativeName | 강성만 | - |
| dc.contributor.alternativeName | 송인성 | - |
| dc.contributor.alternativeName | 이혜영 | - |
| dc.contributor.alternativeName | 나도선 | - |
| dc.identifier.bibliographicCitation | Korean Biochemical Journal, vol. 23, no. 4, pp. 459-464 | - |
| dc.description.journalClass | N | - |
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