Effects of interleukin-4(B cell stimulatory factor-1) on B cell growth and differentiation = Interleukin-4(B cell stimulatory factor-1)의 B임파구 성장 및 분화에 대한 작용

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dc.contributor.authorSuk Ran Yoon-
dc.contributor.authorSu Yeon Kim-
dc.contributor.authorChoong Eun Lee-
dc.contributor.authorTai Wha Chung-
dc.contributor.authorKwang Ho Pyun-
dc.description.abstractInterleukin-4(IL-4), a 20,000 dalton glycoprotein produced by activated T-cells, has been re-port-ed to have pleiotropic effects on immune and hematopoietic systems. We studied various biologi-cal effects of IL-4 on the growth of B-cells and T-cells as well as on the B-cell differentiation and maturation. Using [3H] thymidine incorporation as an index for B-cell proliferation, we performed IL-4 bioassay employing anti-IgM costimulation method. Human recombinant IL?4 induced active proliferation of purified tonsillar B cells in the presence of insolubilized anti-IgM antibody at the optimal concentration of 5신g/ml. The soluble anti-IgM was ineffective. By examining the ability of T cell culture supernatant to induce B-cell proliferation, the optimal culture condition of T-cells for the effective IL-4 production was found to be the combined treatment with PHA lO/eg/ml and PMA 20ng/ml. IL-4 increased the expression of membrane FceR H (low affinity receptor for IgE: CD23) and soluble FceR n in a dose-dependent manner indicating that IL-4 could be involved in IgE production and immediate type hypersensitivity. In contrast to the reported effects of IL-4 on the immunoglobulin production and IgGi isotype switch in the murine system, we did not observe such effects with the human system, which may require more sensitive assay methods. IL-4 also induced proliferation of activated tonsillar T-cells but did not have any effects on resting T-cells. Detailed elucidation of mechanisms underlying these various biological activities of IL-4 would require the characterization, purification and molecular cloning of IL-4 receptor.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleEffects of interleukin-4(B cell stimulatory factor-1) on B cell growth and differentiation = Interleukin-4(B cell stimulatory factor-1)의 B임파구 성장 및 분화에 대한 작용-
dc.title.alternativeEffects of interleukin-4(B cell stimulatory factor-1) on B cell growth and differentiation-
dc.citation.titleKorean Journal of Immunology-
dc.contributor.affiliatedAuthorSuk Ran Yoon-
dc.contributor.affiliatedAuthorChoong Eun Lee-
dc.contributor.affiliatedAuthorTai Wha Chung-
dc.contributor.affiliatedAuthorKwang Ho Pyun-
dc.identifier.bibliographicCitationKorean Journal of Immunology, vol. 12, no. 1, pp. 41-51-
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Division of Biomedical Research > Immunotherapy Research Center > 1. Journal Articles
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