TNF-α secreted from macrophages increases the expression of prometastatic integrin αV in gastric cancer

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dc.contributor.authorMi Aie Hwang-
dc.contributor.authorMisun Won-
dc.contributor.authorJoo-Young Im-
dc.contributor.authorMi-Jung Kang-
dc.contributor.authorD H Kweon-
dc.contributor.authorBo Kyung Kim-
dc.date.accessioned2023-01-02T16:32:58Z-
dc.date.available2023-01-02T16:32:58Z-
dc.date.issued2023-
dc.identifier.issn1661-6596-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/30843-
dc.description.abstractThe tumor microenvironment comprising blood vessels, fibroblasts, immune cells, and the extracellular matrix surrounding cancer cells, has recently been targeted for research in cancer therapy. We aimed to investigate the effect of macrophages on the invasive ability of gastric cancer cells, and studied their potential mechanism. In transcriptome analysis, integrin αV was identified as a gene increased in AGS cells cocultured with RAW264.7 cells. AGS cells cocultured with RAW264.7 cells displayed increased adhesion to the extracellular matrix and greater invasiveness compared with AGS cells cultured alone. This increased invasion of AGS cells cocultured with RAW264.7 cells was inhibited by integrin αV knockdown. In addition, the increase in integrin αV expression induced by tumor necrosis factor-α (TNF-α) or by coculture with RAW264.7 cells was inhibited by TNF receptor 1 (TNFR1) knockdown. The increase in integrin αV expression induced by TNF-α was inhibited by both Mitogen-activated protein kinase (MEK) inhibitor and VGLL1 S84 peptide treatment. Finally, transcription of integrin αV was shown to be regulated through the binding of VGLL1 and TEAD4 to the promoter of integrin αV. In conclusion, our study demonstrated that TNFR1?ERK?VGLL1 signaling activated by TNF-α secreted from RAW264.7 cells increased integrin αV expression, thereby increasing the adhesion and invasive ability of gastric cancer cells.-
dc.publisherMDPI-
dc.titleTNF-α secreted from macrophages increases the expression of prometastatic integrin αV in gastric cancer-
dc.title.alternativeTNF-α secreted from macrophages increases the expression of prometastatic integrin αV in gastric cancer-
dc.typeArticle-
dc.citation.titleInternational Journal of Molecular Sciences-
dc.citation.number1-
dc.citation.endPage376-
dc.citation.startPage376-
dc.citation.volume24-
dc.contributor.affiliatedAuthorMi Aie Hwang-
dc.contributor.affiliatedAuthorMisun Won-
dc.contributor.affiliatedAuthorJoo-Young Im-
dc.contributor.affiliatedAuthorMi-Jung Kang-
dc.contributor.affiliatedAuthorBo Kyung Kim-
dc.contributor.alternativeName황미애-
dc.contributor.alternativeName원미선-
dc.contributor.alternativeName임주영-
dc.contributor.alternativeName강미정-
dc.contributor.alternativeName권대혁-
dc.contributor.alternativeName김보경-
dc.identifier.bibliographicCitationInternational Journal of Molecular Sciences, vol. 24, no. 1, pp. 376-376-
dc.identifier.doi10.3390/ijms24010376-
dc.subject.keywordGastric cancer-
dc.subject.keywordMacrophage-
dc.subject.keywordTNF-α-
dc.subject.keywordIntegrin αV-
dc.subject.localGastric cancer-
dc.subject.localGastric cancer (GC)-
dc.subject.localgastric cancer-
dc.subject.localmacrophage-
dc.subject.localmacrophages-
dc.subject.localMacrophage-
dc.subject.localMacrophages-
dc.subject.localTNF-a-
dc.subject.localTNF-alpha-
dc.subject.localTNF-α-
dc.subject.localTNFa-
dc.subject.localTNFα-
dc.subject.localTnf-α-
dc.subject.localTumor necrosis fa tor-α-
dc.subject.localTumor necrosis factor (TNF)-α-
dc.subject.localTumor necrosis factor alpha-
dc.subject.localTumor necrosis factor-alpha-
dc.subject.localTumor necrosis factor-α-
dc.subject.localtumor necrosis factor-alpha-
dc.subject.localtumor necrosis factor-α-
dc.description.journalClassY-
Appears in Collections:
Division of Biomedical Research > Personalized Genomic Medicine Research Center > 1. Journal Articles
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