Dual-functional carboxymethyl levan-based protein carrier for cosmeceutical application of human epidermal growth factor

Cited 2 time in scopus
Metadata Downloads

Full metadata record

DC FieldValueLanguage
dc.contributor.authorHyunjun Ko-
dc.contributor.authorBong Hyun Sung-
dc.contributor.authorMi-Jin Kim-
dc.contributor.authorH J Park-
dc.contributor.authorJung Hoon Sohn-
dc.contributor.authorJung Hoon Bae-
dc.date.accessioned2023-01-03T16:32:29Z-
dc.date.available2023-01-03T16:32:29Z-
dc.date.issued2023-
dc.identifier.issn0141-8130-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/30845-
dc.description.abstractHuman epidermal growth factor (hEGF) has been a subject of extensive research as its wide range of physiological functions has many potential applications. However, due to the low stability of hEGF, its physiological effect is easily lost under conditions of use. To compensate for this, we developed a stable delivery system using levan-based nanoparticles. The entrapment yield of various tested proteins was significantly improved by employing carboxymethyl levan (CML) instead of levan; the entrapment yield of the CML-hEGF nanoparticles was 84.1 %. The size and zeta potential of the nanoparticles were identified as 199.9 ± 3.87 nm and -19.1 mV, respectively, using scanning electron microscopy (SEM) and particle size analysis. Dual biological functions of the nanoparticles (skin regeneration and moisturizing) were identified through collagen synthesis activity and aquaporin 3 expression level analysis. Stability of the prepared nanoparticles was also investigated via cell proliferation activity comparison under mimicked physiological conditions. The CML-hEGF nanoparticles maintained cell proliferation activity over 100 % for 6 weeks, while free hEGF was almost inactivated within 2 weeks. Taken together, our results indicate that the CML-based hEGF nanoparticles can be used in pharma- and cosmeceutical applications, guaranteeing a high entrapment capability, functionality, and stability.-
dc.publisherElsevier-
dc.titleDual-functional carboxymethyl levan-based protein carrier for cosmeceutical application of human epidermal growth factor-
dc.title.alternativeDual-functional carboxymethyl levan-based protein carrier for cosmeceutical application of human epidermal growth factor-
dc.typeArticle-
dc.citation.titleInternational Journal of Biological Macromolecules-
dc.citation.number0-
dc.citation.endPage187-
dc.citation.startPage181-
dc.citation.volume229-
dc.contributor.affiliatedAuthorHyunjun Ko-
dc.contributor.affiliatedAuthorBong Hyun Sung-
dc.contributor.affiliatedAuthorMi-Jin Kim-
dc.contributor.affiliatedAuthorJung Hoon Sohn-
dc.contributor.affiliatedAuthorJung Hoon Bae-
dc.contributor.alternativeName고현준-
dc.contributor.alternativeName성봉현-
dc.contributor.alternativeName김미진-
dc.contributor.alternativeName박현주-
dc.contributor.alternativeName손정훈-
dc.contributor.alternativeName배정훈-
dc.identifier.bibliographicCitationInternational Journal of Biological Macromolecules, vol. 229, pp. 181-187-
dc.identifier.doi10.1016/j.ijbiomac.2022.12.278-
dc.subject.keywordLevan-
dc.subject.keywordCarboxymethylation-
dc.subject.keywordEpidermal growth factor (EGF)-
dc.subject.keywordNanoparticle-
dc.subject.keywordStability-
dc.subject.keywordDelivery system-
dc.subject.localLevan-
dc.subject.locallevan-
dc.subject.localEpidermal growth factor-
dc.subject.localEpidermal growth factor (EGF)-
dc.subject.localNanoparticle-
dc.subject.localNanoparticles-
dc.subject.localnanoparticle-
dc.subject.localStability-
dc.subject.localstability-
dc.description.journalClassY-
Appears in Collections:
Synthetic Biology and Bioengineering Research Institute > Synthetic Biology Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.