Magnetic-bead-based DNA-capture-assisted real-time polymerase chain reaction and recombinase polymerase amplification for the detection of African swine fever virus

Cited 2 time in scopus
Metadata Downloads

Full metadata record

DC FieldValueLanguage
dc.contributor.authorG Dhandapani-
dc.contributor.authorV G Nguyen-
dc.contributor.authorM C Kim-
dc.contributor.authorJ Y Noh-
dc.contributor.authorS S Jang-
dc.contributor.authorSun Woo Yoon-
dc.contributor.authorDae Gwin Jeong-
dc.contributor.authorT M L-
dc.contributor.authorV P Le-
dc.contributor.authorD Song-
dc.contributor.authorH K Kim-
dc.date.accessioned2023-01-04T16:32:49Z-
dc.date.available2023-01-04T16:32:49Z-
dc.date.issued2023-
dc.identifier.issn0304-8608-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/30848-
dc.description.abstractAfrican swine fever (ASF) is a deadly disease in swine caused by African swine fever virus (ASFV). The global spread of ASFV has resulted in significant economic losses worldwide. Improved early detection has been the most important first line of defense for preventing ASF outbreaks and for activating control measures. Despite the availability of rapid amplification methods, nucleic acid extraction from specimens still needs to be performed in a laboratory. To facilitate this step, we exploited the strong affinity of biotin-streptavidin binding by functionalizing streptavidin-coated magnetic beads with biotinylated oligonucleotide capture probes to efficiently capture genotype II ASFV DNA directly from crude clinical samples. The captured DNA is suitable for detection using real-time quantitative PCR (qPCR) and recombinase polymerase amplification (RPA). In this study, ASFV DNA was efficiently captured from swine feces, serum, and tissue samples. Both DNA-capture-assisted qPCR and RPA-based detection methods have a limit of detection (LOD) of 102 copies/μl, which is comparable to those of commercially available kits. In addition, an RPA-SYBR Green I method was developed for the immediate visual detection of ASFV DNA, which is time-saving and efficient for resource-limited field settings. In summary, a rapid, versatile, sequence-specific DNA capture method was developed to efficiently capture ASFV DNA from swine clinical samples and subsequent detection by qPCR and RPA, which has the potential to be used for robust screening and surveillance of ASFV and in point-of-care (POC) diagnostics.-
dc.publisherSpringer-
dc.titleMagnetic-bead-based DNA-capture-assisted real-time polymerase chain reaction and recombinase polymerase amplification for the detection of African swine fever virus-
dc.title.alternativeMagnetic-bead-based DNA-capture-assisted real-time polymerase chain reaction and recombinase polymerase amplification for the detection of African swine fever virus-
dc.typeArticle-
dc.citation.titleArchives of Virology-
dc.citation.number1-
dc.citation.endPage21-
dc.citation.startPage21-
dc.citation.volume168-
dc.contributor.affiliatedAuthorDae Gwin Jeong-
dc.contributor.alternativeNameDhandapani-
dc.contributor.alternativeNameNguyen-
dc.contributor.alternativeName김민찬-
dc.contributor.alternativeName노지영-
dc.contributor.alternativeName장성식-
dc.contributor.alternativeName윤선우-
dc.contributor.alternativeName정대균-
dc.contributor.alternativeNameHuynh-
dc.contributor.alternativeNameLe-
dc.contributor.alternativeName송대섭-
dc.contributor.alternativeName김혜권-
dc.identifier.bibliographicCitationArchives of Virology, vol. 168, no. 1, pp. 21-21-
dc.identifier.doi10.1007/s00705-022-05681-7-
dc.description.journalClassY-
Appears in Collections:
Division of Research on National Challenges > Bionanotechnology Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.