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- Title
- Transcriptional regulation of Notch1 by nuclear factor-κB during T cell activation
- Author(s)
- J R Hwang; D Kim; Jung-Ah Kang; S H Park; S G Park
- Bibliographic Citation
- Scientific Reports, vol. 13, pp. 43-43
- Publication Year
- 2023
- Abstract
- Notch1 plays important roles in T cell development and is highly expressed in activated CD4+ T cells. However, the underlying mechanism of Notch1 transcription in T cells has not been fully characterized. Therefore, we aimed to determine how Notch1 expression is regulated during the activation of CD4+ T cells. Both the surface expression and mRNA transcription of Notch1 were significantly higher in activated CD4+ T cells, but the inhibition of phosphatidylinositol 3-kinase (PI3K) by LY294002 or deletion of the Pdk1 gene impaired this upregulation of Notch1. Interrogation of the Notch1 promoter region using serially deleted Notch1 promoter reporters revealed that the - 300 to - 270 region is crucial for its transcription in activated T cells. In addition, we found that nuclear factor (NF)-κB subunits containing RelA bind directly to this promoter region, thereby upregulating transcription. In addition, inhibition of NF-κB by SN50 impaired upregulation of Notch1 surface protein and mRNA in activated CD4+ T cells. Thus, we provide evidence that Notch1 transcription in activated CD4+ T cells is upregulated via the PI3K-PDK1-NF-κB signaling pathway.
- ISSN
- 2045-2322
- Publisher
- Springer-Nature Pub Group
- DOI
- http://dx.doi.org/10.1038/s41598-022-26674-1
- Type
- Article
- Appears in Collections:
- Division of Research on National Challenges > Bionanotechnology Research Center > 1. Journal Articles
- Files in This Item:
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