Field trials of carrot hydrogel artificial seeds = 당근 수화겔 인공종자의 포장적응

Abstract

A field test was carried out to determine acclimatizing conditions of carrot hydrogel artificial seeds( alginate-encapsulated somatic embryos). Sand soil or vermiculite was put into 500n^ beakers, and true or artificial seeds were sown on its surface. The beakers were covered with transparent vinyl-film and hurried in the field so that the level of sand soil or vermiculite in them was equal to that of the ground. Fifteen days after sowing, 55 究 of the true seeds sown on non-autoclaved sand soil germinated to grow into seedlings, whereas 20쩟 of the artificial seeds converted into plantlets. In addition, plantlets were obtained from 41% of the artificial seeds sown on autoclaved sand soil and 75% on autoclaved vermiculite with one-third strength of Murashige and Skoog's medium, respectively. Usually fungal contamination caused artificial seeds not to convert into plantlets. These results and the preliminary field tests indicate that the survival of artificial seeds in the field conditions depend mostly upon contamination, if a high humidity condition is provided, and that it is not significantly affected by temperature variation in the field.