Development of nucleocapsid-specific monoclonal antibodies for SARS-CoV-2 and their ELISA diagnostics on an automatic microfluidic device

Cited 5 time in scopus
Metadata Downloads

Full metadata record

DC FieldValueLanguage
dc.contributor.authorJihyun Yang-
dc.contributor.authorV M Phan-
dc.contributor.authorChang-Kyu Heo-
dc.contributor.authorH V Nguyen-
dc.contributor.authorWon-Hee Lim-
dc.contributor.authorEun Wie Cho-
dc.contributor.authorHaryoung Poo-
dc.contributor.authorT S Seo-
dc.date.accessioned2023-01-18T16:32:21Z-
dc.date.available2023-01-18T16:32:21Z-
dc.date.issued2023-
dc.identifier.issn0925-4005-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/30905-
dc.description.abstractCoronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection has threatened public health globally, and the emergence of viral variants has exacerbated an already precarious situation. To prevent further spread of the virus and determine government action required for virus control, accurate and rapid immunoassays for SARS-CoV-2 diagnosis are urgently needed. In this study, we generated monoclonal antibodies (mAbs) against the SARS-CoV-2 nucleocapsid protein (NP), compared their reactivity using an enzyme-linked immunosorbent assay (ELISA), and selected four mAbs designated 1G6, 3E10, 3F10, and 5B6 which have higher reactivity to NP and viral lysates of SARS-CoV-2 than other mAbs. Using an epitope mapping assay, we identified that 1G6 detected the C-terminal domain of SARS-CoV-2 NP (residues 248?364), while 3E10 and 3F10 bound to the N-terminal domain (residues 47?174) and 3F10 detected the N-arm region (residues 1?46) of SARS-CoV-2 NP. Based on the epitope study and sandwich ELISA, we selected the 1G6 and 3E10 Abs as an optimal Ab pair and applied them for a microfluidics-based point-of-care (POC) ELISA assay to detect the NPs of SARS-CoV-2 and its variants. The integrated and automatic microfluidic system could operate the serial injection of the sample, the washing solution, the HRP-conjugate antibody, and the TMB substrate solution simply by controlling air purge via a single syringe. The proposed Ab pair-equipped microsystem effectively detected the NPs of SARS-CoV-2 variants as well as in clinical samples. Collectively, our proposed platform provides an advanced protein-based diagnostic tool for detecting SARS-CoV-2.-
dc.publisherElsevier-
dc.titleDevelopment of nucleocapsid-specific monoclonal antibodies for SARS-CoV-2 and their ELISA diagnostics on an automatic microfluidic device-
dc.title.alternativeDevelopment of nucleocapsid-specific monoclonal antibodies for SARS-CoV-2 and their ELISA diagnostics on an automatic microfluidic device-
dc.typeArticle-
dc.citation.titleSensors and Actuators B-Chemical-
dc.citation.number0-
dc.citation.endPage133331-
dc.citation.startPage133331-
dc.citation.volume380-
dc.contributor.affiliatedAuthorJihyun Yang-
dc.contributor.affiliatedAuthorChang-Kyu Heo-
dc.contributor.affiliatedAuthorWon-Hee Lim-
dc.contributor.affiliatedAuthorEun Wie Cho-
dc.contributor.affiliatedAuthorHaryoung Poo-
dc.contributor.alternativeName양지현-
dc.contributor.alternativeNamePhan-
dc.contributor.alternativeName허창규-
dc.contributor.alternativeNameNguyen-
dc.contributor.alternativeName임원희-
dc.contributor.alternativeName조은위-
dc.contributor.alternativeName부하령-
dc.contributor.alternativeName서태석-
dc.identifier.bibliographicCitationSensors and Actuators B-Chemical, vol. 380, pp. 133331-133331-
dc.identifier.doi10.1016/j.snb.2023.133331-
dc.subject.keywordSARS-CoV-2-
dc.subject.keywordNucleocapsid-
dc.subject.keywordDiagnosis-
dc.subject.keywordAntibody pair-
dc.subject.keywordMicrofluidic device-
dc.subject.keywordPoint-of-care testing-
dc.subject.localSARS-CoV-2-
dc.subject.localSARS-Cov-2-
dc.subject.localnucleocapsid-
dc.subject.localNucleocapsid-
dc.subject.localdiagnosis-
dc.subject.localDiagnosis-
dc.subject.localAntibody pair-
dc.subject.localmicrofluidic device-
dc.subject.localMicrofluidic device-
dc.subject.localMicrofluidic devices-
dc.subject.localPoint-of-care test-
dc.subject.localPoint-of-care testing-
dc.subject.localPoint-of-care testing (POCT)-
dc.subject.localpoint-of-care testing (POCT)-
dc.subject.localpoint-of-care-testing-
dc.subject.localPoCT-
dc.subject.localpoint of care testing-
dc.subject.localPoint of Care Testing-
dc.subject.localPOCT-
dc.subject.localPoint of care testing-
dc.subject.localPoint-of-care test (POCT)-
dc.description.journalClassY-
Appears in Collections:
Division of Research on National Challenges > Infectious Disease Research Center > 1. Journal Articles
Division of Biomedical Research > Rare Disease Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.