Generation of proximal tubule spheroids for nephrotoxicity assessment

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dc.contributor.authorDae Hun Kim-
dc.contributor.authorJung Hwa Lim-
dc.contributor.authorCho-Rok Jung-
dc.contributor.authorHyun Mi Kang-
dc.date.accessioned2023-01-19T16:32:49Z-
dc.date.available2023-01-19T16:32:49Z-
dc.date.issued2022-
dc.identifier.issn2765-205X-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/30910-
dc.description.abstractBackground: To date, nephrotoxicity in new drug development has been evaluated through two-dimensional culture of representative cell lines, such as HK-2 and human proximal tubule epithelial cells (hPTECs). Approximately 20% of new drugs that were safe in preclinical studies were withdrawn from clinical trials due to nephrotoxicity, which means the current renal cell lines used in preclinical trials have limitations for the accurate detection of nephrotoxicity. Methods and Results: Here, we established proximal tubule cell lines from immortalized mixed primary renal cells and generated functional proximal tubule cell spheroids, which expressed all apical basolateral transporters and showed epithelial polarity. Moreover, they showed a more sensitive drug response than hPTECs, which have been commonly used as in vitro kidney models. Conclusion: Taken together, the proximal tubule cells described in this study provide a more stable, reproducible, and accurate in vitro kidney model for predicting nephrotoxicity, which could help early compound development.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleGeneration of proximal tubule spheroids for nephrotoxicity assessment-
dc.title.alternativeGeneration of proximal tubule spheroids for nephrotoxicity assessment-
dc.typeArticle-
dc.citation.titleOrganoid-
dc.citation.number0-
dc.citation.endPagee30-
dc.citation.startPagee30-
dc.citation.volumee-
dc.contributor.affiliatedAuthorDae Hun Kim-
dc.contributor.affiliatedAuthorJung Hwa Lim-
dc.contributor.affiliatedAuthorCho-Rok Jung-
dc.contributor.affiliatedAuthorHyun Mi Kang-
dc.contributor.alternativeName김대훈-
dc.contributor.alternativeName임정화-
dc.contributor.alternativeName정초록-
dc.contributor.alternativeName강현미-
dc.identifier.bibliographicCitationOrganoid, vol. e, pp. e30-e30-
dc.identifier.doi10.51335/organoid.2022.2.e30-
dc.subject.keywordProximal tubule cells-
dc.subject.keywordIn vitro kidney model-
dc.subject.keywordNephrotoxicity-
dc.subject.keywordKidney spheroids-
dc.subject.localNephrotoxicity-
dc.subject.localnephrotoxicity-
dc.description.journalClassN-
Appears in Collections:
Division of Research on National Challenges > Stem Cell Convergenece Research Center > 1. Journal Articles
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