Molecular properties of global suppressors of temperature-sensitive folding mutations in P22 tailspike endorhamnosidase

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Title
Molecular properties of global suppressors of temperature-sensitive folding mutations in P22 tailspike endorhamnosidase
Author(s)
Sang Chul Lee; Heyeong Koh; Myeong Hee Yu
Bibliographic Citation
Journal of Biological Chemistry, vol. 266, no. 34, pp. 23191-23196
Publication Year
1991
Abstract
Two global suppressors (Val-331 > Ala and Ala-334 > Val) have been identified for temperature-sensitive folding (tsf) mutations in gene 9 of bacteriophage P22 (Mitraki, A., Fane, B., Haase-Pettingell, C., Sturtevant, J., and King, J. (1991) Science 253, 54-58). We have introduced 19 different single amino acid substitutions at the two global suppressor sites independently and examined the effects on the tailspike formation in Escherichia coli. Folding and maturation patterns of the various substitutions at the two global suppressor sites in the wild-type background suggest that Val-331 is located on the protein surface and Ala-334 is in the hydrophobic region. In combination with a tsf mutation, tsfH304 (Gly-244 > Arg), only Gly at 331 and Ile at 334, the substitutions that have similar side chain properties to the original suppressor sequences, were active as tsf suppressors. The newly identified suppressors of tsfH304 could also alleviate the tsf defect of three other mutations. The mutant carrying both Val-331 > Ala and Ala-334 > Val substitutions was also a global suppressor and was more active in suppressing the tsf defect than mutants carrying only one substitution. The suppressors may act by increasing the stability of an intermediate in the productive pathway of folding and maturation of the mutant polypeptides.
Keyword
suppressor factorbacteriophage p22protein foldingtemperature sensitivityvirus mutationtemperature
ISSN
0021-9258
Publisher
Amer Soc Biochemistry Molecular Biology Inc
Type
Article
Appears in Collections:
Division of Biomedical Research > Metabolic Regulation Research Center > 1. Journal Articles
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