DC Field | Value | Language |
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dc.contributor.author | S S Yim | - |
dc.contributor.author | J W Choi | - |
dc.contributor.author | Yong Jae Lee | - |
dc.contributor.author | K J Jeong | - |
dc.date.accessioned | 2023-02-23T16:32:40Z | - |
dc.date.available | 2023-02-23T16:32:40Z | - |
dc.date.issued | 2023 | - |
dc.identifier.issn | 1475-2859 | - |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/31104 | - |
dc.description.abstract | Background: The disposal of plastic waste is a major environmental challenge. With recent advances in microbial genetic and metabolic engineering technologies, microbial polyhydroxyalkanoates (PHAs) are being used as next-generation biomaterials to replace petroleum-based synthetic plastics in a sustainable future. However, the relatively high production cost of bioprocesses hinders the production and application of microbial PHAs on an industrial scale. Results: Here, we describe a rapid strategy to rewire metabolic networks in an industrial microorganism, Corynebacterium glutamicum, for the enhanced production of poly(3-hydroxybutyrate) (PHB). A three-gene PHB biosynthetic pathway in Rasltonia eutropha was refactored for high-level gene expression. A fluorescence-based quantification assay for cellular PHB content using BODIPY was devised for the rapid fluorescence-activated cell sorting (FACS)-based screening of a large combinatorial metabolic network library constructed in C. glutamicum. Rewiring metabolic networks across the central carbon metabolism enabled highly efficient production of PHB up to 29% of dry cell weight with the highest cellular PHB productivity ever reported in C. glutamicum using a sole carbon source. Conclusions: We successfully constructed a heterologous PHB biosynthetic pathway and rapidly optimized metabolic networks across central metabolism in C. glutamicum for enhanced production of PHB using glucose or fructose as a sole carbon source in minimal media. We expect that this FACS-based metabolic rewiring framework will accelerate strain engineering processes for the production of diverse biochemicals and biopolymers. | - |
dc.publisher | Springer-BMC | - |
dc.title | Rapid combinatorial rewiring of metabolic networks for enhanced poly(3-hydroxybutyrate) production in Corynebacterium glutamicum | - |
dc.title.alternative | Rapid combinatorial rewiring of metabolic networks for enhanced poly(3-hydroxybutyrate) production in Corynebacterium glutamicum | - |
dc.type | Article | - |
dc.citation.title | Microbial Cell Factories | - |
dc.citation.number | 0 | - |
dc.citation.endPage | 29 | - |
dc.citation.startPage | 29 | - |
dc.citation.volume | 22 | - |
dc.contributor.affiliatedAuthor | Yong Jae Lee | - |
dc.contributor.alternativeName | 임성순 | - |
dc.contributor.alternativeName | 최재웅 | - |
dc.contributor.alternativeName | 이용재 | - |
dc.contributor.alternativeName | 정기준 | - |
dc.identifier.bibliographicCitation | Microbial Cell Factories, vol. 22, pp. 29-29 | - |
dc.identifier.doi | 10.1186/s12934-023-02037-x | - |
dc.subject.keyword | Corynebacterium glutamicum | - |
dc.subject.keyword | Metabolic engineering | - |
dc.subject.keyword | Combinatorial optimization | - |
dc.subject.keyword | Poly(3-hydroxybutyrate) | - |
dc.subject.local | Corynebacterium glutamicum | - |
dc.subject.local | corynebacterium glutamicum | - |
dc.subject.local | metabolic engineering | - |
dc.subject.local | Metabolic engineering | - |
dc.subject.local | Metabolic Engineering | - |
dc.subject.local | Combinatorial optimization | - |
dc.subject.local | Poly(3-hydroxybutyrate) | - |
dc.subject.local | poly(3-hydroxybutyrate) | - |
dc.description.journalClass | Y | - |
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