Rapid combinatorial rewiring of metabolic networks for enhanced poly(3-hydroxybutyrate) production in Corynebacterium glutamicum

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dc.contributor.authorS S Yim-
dc.contributor.authorJ W Choi-
dc.contributor.authorYong Jae Lee-
dc.contributor.authorK J Jeong-
dc.date.accessioned2023-02-23T16:32:40Z-
dc.date.available2023-02-23T16:32:40Z-
dc.date.issued2023-
dc.identifier.issn1475-2859-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/31104-
dc.description.abstractBackground: The disposal of plastic waste is a major environmental challenge. With recent advances in microbial genetic and metabolic engineering technologies, microbial polyhydroxyalkanoates (PHAs) are being used as next-generation biomaterials to replace petroleum-based synthetic plastics in a sustainable future. However, the relatively high production cost of bioprocesses hinders the production and application of microbial PHAs on an industrial scale. Results: Here, we describe a rapid strategy to rewire metabolic networks in an industrial microorganism, Corynebacterium glutamicum, for the enhanced production of poly(3-hydroxybutyrate) (PHB). A three-gene PHB biosynthetic pathway in Rasltonia eutropha was refactored for high-level gene expression. A fluorescence-based quantification assay for cellular PHB content using BODIPY was devised for the rapid fluorescence-activated cell sorting (FACS)-based screening of a large combinatorial metabolic network library constructed in C. glutamicum. Rewiring metabolic networks across the central carbon metabolism enabled highly efficient production of PHB up to 29% of dry cell weight with the highest cellular PHB productivity ever reported in C. glutamicum using a sole carbon source. Conclusions: We successfully constructed a heterologous PHB biosynthetic pathway and rapidly optimized metabolic networks across central metabolism in C. glutamicum for enhanced production of PHB using glucose or fructose as a sole carbon source in minimal media. We expect that this FACS-based metabolic rewiring framework will accelerate strain engineering processes for the production of diverse biochemicals and biopolymers.-
dc.publisherSpringer-BMC-
dc.titleRapid combinatorial rewiring of metabolic networks for enhanced poly(3-hydroxybutyrate) production in Corynebacterium glutamicum-
dc.title.alternativeRapid combinatorial rewiring of metabolic networks for enhanced poly(3-hydroxybutyrate) production in Corynebacterium glutamicum-
dc.typeArticle-
dc.citation.titleMicrobial Cell Factories-
dc.citation.number0-
dc.citation.endPage29-
dc.citation.startPage29-
dc.citation.volume22-
dc.contributor.affiliatedAuthorYong Jae Lee-
dc.contributor.alternativeName임성순-
dc.contributor.alternativeName최재웅-
dc.contributor.alternativeName이용재-
dc.contributor.alternativeName정기준-
dc.identifier.bibliographicCitationMicrobial Cell Factories, vol. 22, pp. 29-29-
dc.identifier.doi10.1186/s12934-023-02037-x-
dc.subject.keywordCorynebacterium glutamicum-
dc.subject.keywordMetabolic engineering-
dc.subject.keywordCombinatorial optimization-
dc.subject.keywordPoly(3-hydroxybutyrate)-
dc.subject.localCorynebacterium glutamicum-
dc.subject.localcorynebacterium glutamicum-
dc.subject.localmetabolic engineering-
dc.subject.localMetabolic engineering-
dc.subject.localMetabolic Engineering-
dc.subject.localCombinatorial optimization-
dc.subject.localPoly(3-hydroxybutyrate)-
dc.subject.localpoly(3-hydroxybutyrate)-
dc.description.journalClassY-
Appears in Collections:
Synthetic Biology and Bioengineering Research Institute > Cell Factory Research Center > 1. Journal Articles
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