Generation of telomeric repeat binding factor 1 (TRF1)-knockout human embryonic stem cell lines, KRIBBe010-A-95, KRIBBe010-A-96, and KRIBBe010-A-97, using CRISPR/Cas9 technology
Cited 0 time in
- Title
- Generation of telomeric repeat binding factor 1 (TRF1)-knockout human embryonic stem cell lines, KRIBBe010-A-95, KRIBBe010-A-96, and KRIBBe010-A-97, using CRISPR/Cas9 technology
- Author(s)
- Onju Ham; Seongjun Kim; Youngsun Lee; Mi Ok Lee
- Bibliographic Citation
- Stem Cell Research, vol. 68, pp. 103045-103045
- Publication Year
- 2023
- Abstract
- Telomeric repeat binding factor 1 (TRF1) plays an essential role in maintaining telomere length. Here, we established TRF1-knockout human pluripotent stem cells (hPSCs; hTRF1-KO) using the CRISPR/Cas9 technology. The hTRF1-KO cell lines expressed pluripotency markers and demonstrated a normal karyotype (46, XX) and DNA profile. In addition, hTRF1-KOcells spontaneously differentiated into all three germ layers in vitro. Thus, these cell lines could be useful models in various research fields.
- ISSN
- 1873-5061
- Publisher
- Elsevier
- DOI
- http://dx.doi.org/10.1016/j.scr.2023.103045
- Type
- Article
- Appears in Collections:
- Division of Research on National Challenges > Stem Cell Convergenece Research Center > 1. Journal Articles
- Files in This Item:
Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.