Sandwich enzyme-linked immunosorbent assay for interleukin-2 = 인터루킨-2 정량을 위한 효소면역 측정법

Abstract

To establish an enzyme-linked immunosorbent assay technique to quantitate inter-leukin-2 (IL-2), five different monoclonal antibodies against human recombinant IL-2 were prepared. A pair of monoclonal antibodies recognizing different epitopes on the IL-2 molecule were selected for sandwich ELISA. The procedure involved utilization of one specific antibody immobilized on a microtiter plate and the other labelled with alkaline phosphatase. A linear relationship was achieved, using concentrations of IL-2 up to 100 ng/ml, when the absorbance of the reaction mixture was measured on a spectrophotometer. The whole assay procedure can be completed within five h. This assay technique for IL-2 would provide a simple and rapid alternative to other assays such as bioassay using IL-2 dependent CTLL cells.