In vitro induction of human antibody against hepatitis B virus surface antigen = B형 간염 바이러스 표면항원에 대한 항체의 유도

Abstract

Human peripheral blood lymphocytes (PBL) were isolated from the heparinized blood of antibody positive donors by centrifugation with Ficoll/Paque solution. The PBL were transformed with Epstein-Barr virus and the clones were screened for anti-HBsAg antibody production. Twenty-three percent of the total clones were HBsAg antibody positive. Among them, one clone showed stable antibody production. One of the polyclonal B cell activators, pokeweed mitogen, was effective for production of anti-HBsAg antibody and cell proliferation.