Selection of protoplasts-derived cell lines for high yields indole alkaloid from suspension cultures of Vinca(Catharanthus roseus) = 일일초(Catharanthus roseus) 현탁배양으로부터 원형질체유래 indole

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Title
Selection of protoplasts-derived cell lines for high yields indole alkaloid from suspension cultures of Vinca(Catharanthus roseus) = 일일초(Catharanthus roseus) 현탁배양으로부터 원형질체유래 indole
Author(s)
Suk Weon Kim; Kyung Hee Jung; Sang Soo Kwak; H S Choi; C Y Choi; Jang Ryol Liu
Bibliographic Citation
Korean Journal of Biotechnology and Bioengineering, vol. 6, no. 1, pp. 1-7
Publication Year
1991
Abstract
To produce economically important indole alkaloids by cell cultures, we selected protoplasts--derived clc nes (protoclones) of vinca (Catharanthus roseus、for high yields of catharanthine and ajmalicine. Protoplasts were enzymatically isolated from suspension - cultured cells. The highest plating effi iency (1%) was obtained when protoplasts were plated at a density of 1X105 protoplasts / m in a culture medium solidified with 0.4% Seaplaque agarose. The growth rates of 40 protoclc les subcultured on a solid medium varied over a wide range. Protoclone VPC-6, which had she highest growth rate, was observed to produce relatively high yields of catharanthine and ajmalicine when cultured in a liquid medium. Although the original cell line did not produce cat laranthine at a detectable level by HPLC, protoclone VPC-10 produced it at a level of 5.9서g / g fresh weight of cells for 10 days of culture. Under the same conditions, protoclone VPC-15 pr< duced ajmalicine at a level of 133.6a授 /g, of which productivity was improved about 3 times th; n that of the original cell line. The results indicate that differences in the growth rate and ir dole alkaloid yield among the protoclones reflect the somaclonal variation in suspe-nsion-cultu ed cells.
ISSN
1225-7117
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Biological Resource Center > 1. Journal Articles
Division of Research on National Challenges > Plant Systems Engineering Research > 1. Journal Articles
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