Plant regeneration from protoplasts of Panax ginseng (C.A. Meyer) through somatic embryogenesis

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Title
Plant regeneration from protoplasts of Panax ginseng (C.A. Meyer) through somatic embryogenesis
Author(s)
S Arya; Jang Ryol Liu; T Eriksson
Bibliographic Citation
Plant Cell Reports, vol. 10, pp. 277-281
Publication Year
1991
Abstract
Protoplasts of Panax ginseng were isolated from embryos obtained from the 4-year old embryogenic cell line KCTC PCL 49031 which was derived from a zygotic embryo. High protoplast yields of 22-25 × 106 protoplast / g tissue were obtained following 5-6 h digestion with 2% Cellulysin, 1% Pectinase and 1% Macerasae in half strength Murashige and Skoog's medium containing 12% mannitol. A plating density of 1×105 protoplasts /ml was found optimal for protoplast culture. An initial division frequency of 10% was obtained in an agarosegelled defined medium. Myo-inositol (6%) was found to be the most suitable osmoticum. Somatic embryos were formed from protoplast derived embryogenic callus, which regenerated into plantlets.
ISSN
0721-7714
Publisher
Springer
DOI
http://dx.doi.org/10.1007/BF00193141
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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