Dasania phycosphaerae sp. nov., isolated from phytoplankton sample from the south coast of the Republic of Korea

Abstract

A novel bacterial strain, designated as GY-18T, was isolated from particulate material sampled from the Korean south coast. The bacterium was Gram-stain-negative, aerobic and rod-shaped. Oxidase and catalase were positive. Optimal growth was observed at 30 °C, pH 7.0, in the presence of 1% (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene and whole-genome sequences suggested that strain GY-18T was a member of the genus Dasania, but represented a separate lineage. The 16S rRNA gene sequence of strain GY-18T showed high similarities to Dasania marina KCTC 12566T (98.4%), Halioglobus japonicus KCTC 23429T (92.2%), Marimicrobium arenosum KCTC 42300T (92.4%) and Seongchinamella unica KCTC 62383T (92.2%), respectively. The genome length of strain GY-18T was 3808450 bp with a DNA G+C content of 47.8mol%. The major cellular fatty acids of strain GY-18T were summed feature 3 (comprising C16:1 ω7c and/or C16:1 ω6c), C16:0 and C10:0 3-OH (>10%). Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and phospholipids were shown to be the major polar lipids. The predominant isoprenoid quinone was Q-8. Based on phylogenetic, phenotypic, chemotaxonomic and genomic features, we propose that strain GY-18T represents a novel species in the genus Dasania of the family Spongiibacteraceae, with the proposed name Dasania phycosphaerae sp. nov. The type strain is GY-18T (=KCTC 92290T=GDMCC 1.3586T).