Full-term development of ultrarapidly frozen-thawed mouse 2-cell embryos = 초급속 동결융해한 생쥐 2세포기 수정란의 개체발생능

Abstract

This study investigated full-term development potential of ultrarapidly frozen and thawed mouse 2-cell embryos. Mouse 2-cell embryos, dehydrated by exposure to freezing medium, were directly immersed into liquid nitrogen and thawed in 37V water. The embryos that were frozen and thawed were cultured in vitro and transferred to foster mothers to examine there developmental potential. As a result, the frozen-thawed 2-cell embryos developed to blastocysts in vitro as a similar rate as control 2-cell embryos did(씨 vitro 2-cell, 86.4 in uiuo 2-cell, 90.9%; solution control. 89.9%； control, 89.7%). Normal live young were obtained from transfer of frozen-thawed embryos to the oviduct and uterus of pseudopregnant recipients (31.4^56.7%).