Morphological changes of bovine epididymal spermatozoa incubated in chemical defined medium = 인공배양액에서 배양된 우정자의 형태적 변화

Abstract

This experiment was carried out to investigate ultrastructural changes that occurred in bovine epididymal spermatozoa during incubation in a BO medium supplemented with 5mM caffeine. No structural changes were observed under electron microscope in the majority of non -incubated sperm(79.8%) keeping the membrane intact. Structural changes were however observed when spermatozoa were cultured in a BO medium supplemented with 5mM caffeine, which were classified into 4 types, “intact(29.4%)”，Mvesiculated(45.6%)“acrosome lost(17. 8%)” and “degenerated(7.2%)” spermatozoa, respectively. These results indicated that: 1) vesiculation of spermatozoa membrane is normal acrosome reaction and acrosome lost of spermatozoa is dead one: 2) caffeine can induce acrosome reaction of bovine epididymal spermatozoa.