Binding of trans-acting regulatory protein DAL82 to upstream induction sequence of allantoin degradative gene DAL7

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dc.contributor.authorMi Sun Won-
dc.contributor.authorKwang Lae Hoe-
dc.contributor.authorT Cooper-
dc.contributor.authorHyang Sook Yoo-
dc.coverage.temporal20161201-20171130-
dc.date.accessioned2017-04-19T08:44:07Z-
dc.date.available2017-04-19T08:44:07Z-
dc.date.issued1992-
dc.identifier.issn1016-8478-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/3174-
dc.description.abstractIn Saccharomyces cerevisiae, expression of allantoin degradative pathway genes (DAL4, DAL7 and DUR1,2) is induced by allophanate. The regulation of DAL7 gene involves interactions between cw-acting elements, upstream activation sequence (UAS), upstream induction sequence (UIS) and upstream repression sequence (URS), and 分次zw-acting factors coded by the DAL81 and DAL82 genes. In this study, the DAL81 or DAL82 gene was fused with glutathione S-trans免rase (Sj26) gene and expressed in 及 coli, GST-DAL81 and GST-DAL82 proteins produced were tested for their binding activities to the cw-acting DNA elements. Gel retardation assays indicated that DAL-81 protein has no DNA-binding activity with any cw-acting elements. Meanwhile, the N-terminal region of DAL82 protein binds to the UIS element. These results suggest that the: DAL7 gene expression is induced by binding of DAL82 protein to UIS and also by protein-protein interactions between DAL81 protein and other regulatory proteins.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleBinding of trans-acting regulatory protein DAL82 to upstream induction sequence of allantoin degradative gene DAL7-
dc.title.alternativeBinding of trans-acting regulatory protein DAL82 to upstream induction sequence of allantoin degradative gene DAL7-
dc.typeArticle-
dc.citation.titleMolecules and Cells-
dc.citation.number2-
dc.citation.endPage219-
dc.citation.startPage213-
dc.citation.volume2-
dc.contributor.affiliatedAuthorMi Sun Won-
dc.contributor.affiliatedAuthorKwang Lae Hoe-
dc.contributor.affiliatedAuthorHyang Sook Yoo-
dc.contributor.alternativeName원미선-
dc.contributor.alternativeName허광래-
dc.contributor.alternativeNameCooper-
dc.contributor.alternativeName유향숙-
dc.identifier.bibliographicCitationMolecules and Cells, vol. 2, no. 2, pp. 213-219-
dc.description.journalClassY-
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Division of Biomedical Research > Personalized Genomic Medicine Research Center > 1. Journal Articles
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