Cloning and characterization of cDNAs coding for heavy and light chains of a monoclonal antibody specific for pre-S2 antigen of hepatitis B virus

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dc.contributor.authorHyo Jeong Hong-
dc.contributor.authorAe Kyung Kim-
dc.contributor.authorChun Jeih Ryu-
dc.contributor.authorSung Sup Park-
dc.contributor.authorHong Keun Chung-
dc.contributor.authorKi Sun Kwon-
dc.contributor.authorKil Lyong Kim-
dc.contributor.authorJiyoung Kim-
dc.contributor.authorMoon Hi Han-
dc.date.accessioned2017-04-19T08:44:07Z-
dc.date.available2017-04-19T08:44:07Z-
dc.date.issued1992-
dc.identifier.issn0378-1119-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/3179-
dc.description.abstractBinding specificity of a monoclonal antibody (mAb) (κ, γ 2b) H8 which can react with the pre-S2 peptide of hepatitis B virus (HBV) determined by Western blot analyses. From the hybridoma cell line secreting mAb H8, poly(A)+ RNA was prepared and used as a template for cDNA synthesis and cloning. Full-length cDNAs coding for the heavy and κ light chains of the mAb were cloned from the cDNA library and characterized by nucleotide (nt) sequence analyses and N-terminal amino acid sequencing. The sequence analyses revealed that both heavy and light chain-specific cDNAs are functional, and the variable regions of the heavy and light chains are members of mouse heavy chain subgroup III(c) and light chain group I, respectively. Comparison of the nt sequences with mouse immunoglobulin genes listed in the GenBank data base show that the cDNAs have not been previously reported. The cDNAs will be used for the construction of a therapeutic antibody for HBV infection.-
dc.publisherElsevier-
dc.titleCloning and characterization of cDNAs coding for heavy and light chains of a monoclonal antibody specific for pre-S2 antigen of hepatitis B virus-
dc.title.alternativeCloning and characterization of cDNAs coding for heavy and light chains of a monoclonal antibody specific for pre-S2 antigen of hepatitis B virus-
dc.typeArticle-
dc.citation.titleGene-
dc.citation.number0-
dc.citation.endPage335-
dc.citation.startPage331-
dc.citation.volume121-
dc.contributor.affiliatedAuthorHyo Jeong Hong-
dc.contributor.affiliatedAuthorChun Jeih Ryu-
dc.contributor.affiliatedAuthorSung Sup Park-
dc.contributor.affiliatedAuthorKi Sun Kwon-
dc.contributor.affiliatedAuthorKil Lyong Kim-
dc.contributor.affiliatedAuthorMoon Hi Han-
dc.contributor.alternativeName홍효정-
dc.contributor.alternativeName김애경-
dc.contributor.alternativeName류춘제-
dc.contributor.alternativeName박성섭-
dc.contributor.alternativeName정홍근-
dc.contributor.alternativeName권기선-
dc.contributor.alternativeName김길룡-
dc.contributor.alternativeName김지영-
dc.contributor.alternativeName한문희-
dc.identifier.bibliographicCitationGene, vol. 121, pp. 331-335-
dc.identifier.doi10.1016/0378-1119(92)90139-G-
dc.subject.keywordcDNA cloning-
dc.subject.keywordHBV-
dc.subject.keywordimmunoglobulin-
dc.subject.keywordnucleotide sequence-
dc.subject.keywordpassive immunoprophylaxis-
dc.subject.keywordrecombinant DNA-
dc.subject.keywordsurface antigen-
dc.subject.localcDNA cloning-
dc.subject.localHBV-
dc.subject.localimmunoglobulin-
dc.subject.localNucleotide sequence-
dc.subject.localnucleotide sequence-
dc.subject.localpassive immunoprophylaxis-
dc.subject.localRecombinant DNA-
dc.subject.localrecombinant DNA-
dc.subject.localsurface antigen-
dc.description.journalClassY-
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Aging Convergence Research Center > 1. Journal Articles
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