Selection of protoclones for high yields of indole alkaloids from suspension cultures of Catharanthus roseus and the qualitative analysis of the compounds by LC-MS

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Title
Selection of protoclones for high yields of indole alkaloids from suspension cultures of Catharanthus roseus and the qualitative analysis of the compounds by LC-MS
Author(s)
K H Jung; Sang Soo KwakSuk Weon Kim; C Y Choi; G S Heo; Jang Ryol Liu
Bibliographic Citation
Biotechnology Techniques, vol. 6, no. 4, pp. 305-308
Publication Year
1992
Abstract
To produce economically important indole alkaloids by cell culture, we have selected protoclones of Catharanthus roseus for high yields of catharanthine and ajmalicine. Protoplasts were enzymatically isolated from suspension-cultured cells. Protoclone VPC-10 produced catharanthine at 5.9 μg/g fresh wt of cells after 10 days of culture, although the original cell line did not produce it at a level detectable by HPLC. Under the same conditions, protoclone VPC-15 produced ajmalicine at 133.6 μg/g, which was about 3 times the productivity of the original cell line. In addition, the indole alkaloids were qualitatively confirmed by LC-MS.
ISSN
0951-208X
Publisher
Unknown
DOI
http://dx.doi.org/10.1007/BF02439316
Type
Article
Appears in Collections:
Division of Research on National Challenges > Plant Systems Engineering Research > 1. Journal Articles
Jeonbuk Branch Institute > Biological Resource Center > 1. Journal Articles
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