Description of Salinimicrobium tongyeongense sp. nov., isolated from seawater

Abstract

A Gram-stain-negative, non-motile by gliding and moderately halophilic rod-shaped bacterium HN-2-9-2T was isolated from seawater in Tongyeong, Republic of Korea. The strain grew at concentrations of 0.5？7？% (w/v) NaCl, at pH 5.5？8.5 and in a temperature range of 18？45 °C. HN-2-9-2T shared the highest 16S rRNA gene sequence percentage with Salinimicrobium xinjiangense BH206T (98.2？%). The average nucleotide identity (ANI), average amino acid identity (AAI) and digital DNA？DNA hybridisation (dDDH) values between HN-2-9-2T and the S. xinjiangense BH206T were 76.0？%, 81.9？% and 19.7？%, respectively. The genome comprised 3？509？958 bp with a DNA G+C content of 43.0%. HN-2-9-2T contained MK-6 as the sole menaquinone. The predominant fatty acids were iso-C15？:？0, anteiso-C15 : 0, iso-C17？:？0 3-OH, iso-C16？:？0, iso-C15？:？1G and summed feature 9, comprising iso-C17？:？1ω6c/C16？:？1 10-methyl. The polar lipids contained phosphatidylethanolamine, one unidentified phospholipid, two unidentified aminolipids, an unidentified glycolipid and six unidentified lipids. The polyphasic taxonomic properties indicate that the strain represents a novel species within the genus Salinimicrobium, for which the name Salinimicrobium tongyeongense sp. nov. is proposed. The type strain is HN-2-9-2T (=KCTC 82934T=NBRC 115920T).